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Plant Vacuolar Trafficking

Overview of attention for book
Attention for Chapter 8: Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells
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Mentioned by

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2 tweeters

Readers on

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5 Mendeley
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Chapter title
Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells
Chapter number 8
Book title
Plant Vacuolar Trafficking
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7856-4_8
Pubmed ID
Book ISBNs
978-1-4939-7855-7, 978-1-4939-7856-4
Authors

Jorge M. P. Rodrigues, Cátia S. Pereira, Natacha Fontes, Hernâni Gerós, Manuela Côrte-Real, Rodrigues, Jorge M. P., Pereira, Cátia S., Fontes, Natacha, Gerós, Hernâni, Côrte-Real, Manuela

Abstract

A series of optimized protocols to isolate vacuoles from both yeast and plant cells, and to characterize the purified organelles at a functional and structural level, are described. For this purpose, we took advantage of the combined use of cell fractionation techniques with different fluorescence-based approaches namely flow cytometry, fluorescence microscopy and spectrofluorimetry. These protocols altogether constitute valuable tools for the study of vacuole structure and function, as well as for the high-throughput screening of drug libraries to identify new molecules that target the vacuole.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 40%
Student > Bachelor 1 20%
Student > Ph. D. Student 1 20%
Unknown 1 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 60%
Agricultural and Biological Sciences 1 20%
Unknown 1 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 November 2018.
All research outputs
#8,395,656
of 13,941,062 outputs
Outputs from Methods in molecular biology
#2,595
of 8,731 outputs
Outputs of similar age
#152,504
of 273,699 outputs
Outputs of similar age from Methods in molecular biology
#1
of 1 outputs
Altmetric has tracked 13,941,062 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 8,731 research outputs from this source. They receive a mean Attention Score of 2.1. This one has gotten more attention than average, scoring higher than 66% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 273,699 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them