Myogenesis

Table of Contents

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   Book Overview
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   Chapter 1 Isolation and Characterization of Human Fetal Myoblasts
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   Chapter 2 Skeletal Muscle Satellite Cells: Background and Methods for Isolation and Analysis in a Primary Culture System
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   Chapter 3 Isolation of Muscle Stem Cells by Fluorescence Activated Cell Sorting Cytometry
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   Chapter 4 Mouse and Human Mesoangioblasts: Isolation and Characterization from Adult Skeletal Muscles
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   Chapter 5 Direct Electrical Stimulation of Myogenic Cultures for Analysis of Muscle Fiber Type Control
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   Chapter 6 Single Muscle-Fiber Isolation and Culture for Cellular, Molecular, Pharmacological, and Evolutionary Studies
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   Chapter 7 Somite Unit Chronometry to Analyze Teratogen Phase Specificity in the Paraxial Mesoderm
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   Chapter 8 Analysis of Skeletal Muscle Development in Drosophila
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    Chapter 9 Immunocytochemistry to Study Myogenesis in Zebrafish
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    Chapter 10 Immunofluorescent Localization of Proteins in Caenorhabditis elegans Muscle
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    Chapter 11 Resistance loading and signaling assays for oxidative stress in rodent skeletal muscle.
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    Chapter 12 Analysis of skeletal muscle hypertrophy in models of increased loading.
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    Chapter 13 Protein Overexpression in Skeletal Muscle Using Plasmid-Based Gene Transfer to Elucidate Mechanisms Controlling Fiber Size
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    Chapter 14 In vivo measurement of muscle protein synthesis rate using the flooding dose technique.
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    Chapter 15 Recombinant Adeno-Associated Viral Vector Production and Purification
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    Chapter 16 Generation of Lentiviral Vectors for Use in Skeletal Muscle Research
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    Chapter 17 Generating Tamoxifen-Inducible Cre Alleles to Investigate Myogenesis in Mice
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    Chapter 18 Gene Profiling Studies in Skeletal Muscle by Quantitative Real-Time Polymerase Chain Reaction Assay
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    Chapter 19 Analysis of Lipid Profiles in Skeletal Muscles
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    Chapter 20 Proteomic Analysis of Dystrophic Muscle
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    Chapter 21 Detection of Calcium Release via Ryanodine Receptors
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    Chapter 22 Measurement of Calcium Release Due to Inositol Trisphosphate Receptors in Skeletal Muscle
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    Chapter 23 Detection of Calcium Sparks in Intact and Permeabilized Skeletal Muscle Fibers
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    Chapter 24 Analysis of Calcium Transients in Cardiac Myocytes and Assessment of the Sarcoplasmic Reticulum Ca 2+ -ATPase Contribution
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    Chapter 25 Analysis of Muscle Gene Transcription in Cultured Skeletal Muscle Cells
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    Chapter 26 Analysis of Fiber-Type Differences in Reporter Gene Expression of β-Gal Transgenic Muscle
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    Chapter 27 Determination of gene promoter activity in skeletal muscles in vivo.
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    Chapter 28 Determination of MiRNA Targets in Skeletal Muscle Cells
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    Chapter 29 shRNA-Mediated Gene Knockdown in Skeletal Muscle
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    Chapter 30 Detection of NF-κB Activity in Skeletal Muscle Cells by Electrophoretic Mobility Shift Analysis
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    Chapter 31 Isolation of Nuclei from Skeletal Muscle Satellite Cells and Myofibers for Use in Chromatin Immunoprecipitation Assays
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    Chapter 32 An Improved Restriction Enzyme Accessibility Assay for Analyzing Changes in Chromatin Structure in Samples of Limited Cell Number
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    Chapter 33 ChIP-Enriched in Silico Targets (ChEST), a ChIP-on-Chip Approach Applied to Analyzing Skeletal Muscle Genes