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Fundamentals and Application of New Bioproduction Systems

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Attention for Chapter 185: Cell-Free Systems: Functional Modules for Synthetic and Chemical Biology
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Chapter title
Cell-Free Systems: Functional Modules for Synthetic and Chemical Biology
Chapter number 185
Book title
Fundamentals and Application of New Bioproduction Systems
Published in
Advances in biochemical engineering biotechnology, April 2013
DOI 10.1007/10_2013_185
Pubmed ID
Book ISBNs
978-3-64-241520-3, 978-3-64-241521-0
Authors

Marlitt Stech, Andreas K. Brödel, Robert B. Quast, Rita Sachse, Stefan Kubick, Andreas K. Brödel

Abstract

: The main goal of cell-free protein synthesis is to produce correctly folded and functional proteins in reasonable amounts for further downstream applications. Especially for eukaryotic proteins, functionality is often directly linked to the presence of posttranslational modifications. Thus, it is of highest interest to develop novel cell-free expression systems that enable the synthesis of posttranslationally modified proteins. Here we present recent advances for the synthesis of glycoproteins, proteins containing disulfide bridges, membrane proteins, and fluorescently labeled proteins. The basis for the expression of these difficult-to-express target proteins is a translationally active cell extract which can be prepared from eukaryotic cell lines such as Spodoptera frugiperda 21 (Sf21) and Chinese hamster ovary (CHO) cells. Due to a very mild lysate preparation procedure, microsomal vesicles derived from the endoplasmic reticulum (ER) can be maintained in the eukaryotic lysate. These vesicles are translocationally active and serve as functional modules facilitating protein translocation and enrichment as well as posttranslational modification of de novo synthesized proteins. In particular, for the synthesis of membrane proteins microsomal vesicles are the essential prerequisite for the insertion of the desired protein into a biologically active membrane scaffold providing a natural environment. We anticipate that the use of such translationally active eukaryotic cell lysates containing translocationally active vesicles may solve a large number of problems still persistent when expressing eukaryotic proteins in vitro.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 17%
Student > Ph. D. Student 4 14%
Student > Master 4 14%
Student > Bachelor 4 14%
Other 3 10%
Other 4 14%
Unknown 5 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 38%
Biochemistry, Genetics and Molecular Biology 10 34%
Medicine and Dentistry 1 3%
Engineering 1 3%
Unknown 6 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 April 2013.
All research outputs
#4,607,747
of 6,230,503 outputs
Outputs from Advances in biochemical engineering biotechnology
#65
of 133 outputs
Outputs of similar age
#65,514
of 97,482 outputs
Outputs of similar age from Advances in biochemical engineering biotechnology
#5
of 10 outputs
Altmetric has tracked 6,230,503 research outputs across all sources so far. This one is in the 15th percentile – i.e., 15% of other outputs scored the same or lower than it.
So far Altmetric has tracked 133 research outputs from this source. They receive a mean Attention Score of 1.5. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 97,482 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.