| Chapter title |
Selective Silencing of Gene Target Expression By siRNA Expression Plasmids in Human Cervical Cancer Cells.
|
|---|---|
| Chapter number | 11 |
| Book title |
Cervical Cancer
|
| Published in |
Methods in molecular biology, October 2014
|
| DOI | 10.1007/978-1-4939-2013-6_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2012-9, 978-1-4939-2013-6
|
| Authors |
Oscar Peralta-Zaragoza, Faustino De-la-O-Gómez, Jessica Deas, Gloria Fernández-Tilapa, Geny Del Socorro Fierros-Zárate, Claudia Gómez-Cerón, Ana Burguete-García, Kirvis Torres-Poveda, Victor Hugo Bermúdez-Morales, Mauricio Rodríguez-Dorantes, Carlos Pérez-Plasencia, Vicente Madrid-Marina, Peralta-Zaragoza, Oscar, De-la-O-Gómez, Faustino, Deas, Jessica, Fernández-Tilapa, Gloria, Fierros-Zárate, Geny del Socorro, Gómez-Cerón, Claudia, Burguete-García, Ana, Torres-Poveda, Kirvis, Bermúdez-Morales, Victor Hugo, Rodríguez-Dorantes, Mauricio, Pérez-Plasencia, Carlos, Madrid-Marina, Vicente, Geny del Socorro Fierros-Zárate |
| Abstract |
RNA interference is a natural mechanism to silence post-transcriptional gene expression in eukaryotic cells in which microRNAs act to cleave or halt the translation of target mRNAs at specific target sequences. Mature microRNAs, 19-25 nucleotides in length, mediate their effect at the mRNA level by inhibiting translation, or inducing cleavage of the mRNA target. This process is directed by the degree of complementary nucleotides between the microRNAs and the target mRNA; perfect complementary base pairing induces cleavage of mRNA, whereas several mismatches lead to translational arrest. Biological effects of microRNAs can be manipulated through the use of small interference RNAs (siRNAs) generated by chemical synthesis, or by cloning in molecular vectors. The cloning of a DNA insert in a molecular vector that will be transcribed into the corresponding siRNAs is an approach that has been developed using siRNA expression plasmids. These vectors contain DNA inserts designed with software to generate highly efficient siRNAs which will assemble into RNA-induced silencing complexes (RISC), and silence the target mRNA. In addition, the DNA inserts may be contained in cloning cassettes, and introduced in other molecular vectors. In this chapter we describe an attractive technology platform to silence cellular gene expression using specific siRNA expression plasmids, and evaluate its biological effect on target gene expression in human cervical cancer cells. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 5% |
| Unknown | 18 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 21% |
| Student > Ph. D. Student | 4 | 21% |
| Researcher | 3 | 16% |
| Student > Master | 2 | 11% |
| Professor | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 4 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 37% |
| Agricultural and Biological Sciences | 3 | 16% |
| Medicine and Dentistry | 3 | 16% |
| Engineering | 1 | 5% |
| Unknown | 5 | 26% |