Title |
Castalagin from Anogeissus leiocarpus mediates the killing of Leishmania in vitro
|
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Published in |
Parasitology Research, August 2008
|
DOI | 10.1007/s00436-008-1137-7 |
Pubmed ID | |
Authors |
M. N. Shuaibu, K. Pandey, P. A. Wuyep, T. Yanagi, K. Hirayama, A. Ichinose, T. Tanaka, I. Kouno |
Abstract |
Stem barks of Anogeissus leiocarpus and Terminalia avicennoides widely used in Africa for treatment of some parasitic diseases were collected and made into methanolic extracts. The extracts were tested on four strains of promastigote forms of Leishmania in vitro. Solvent fractionation in aqueous, butanolic, and ethyl acetate layer indicated butanol and aqueous fractions to have a superior leishmanicidal activity. Chromatographic separation of the butanolic fraction on Sephadex LH-20 followed by nuclear magnetic resonance and correlation high-performance liquid chromatography revealed the presence of known hydrolyzable tannins and some related compounds-with castalagin as the major compound. The observed activity ranged from 62.5 to > or =150, 112.5 to > or =500, and 55 to >150 microg/ml for the crude methanolic extract, different solvent fractions, and the isolated compounds, respectively, on the four different Leishmania strains. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Brazil | 1 | 2% |
Unknown | 54 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 14 | 25% |
Researcher | 6 | 11% |
Student > Master | 6 | 11% |
Student > Doctoral Student | 5 | 9% |
Student > Bachelor | 3 | 5% |
Other | 8 | 15% |
Unknown | 13 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 11 | 20% |
Pharmacology, Toxicology and Pharmaceutical Science | 9 | 16% |
Chemistry | 5 | 9% |
Medicine and Dentistry | 3 | 5% |
Biochemistry, Genetics and Molecular Biology | 3 | 5% |
Other | 6 | 11% |
Unknown | 18 | 33% |