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bcpmr1 encodes a P-type Ca2+/Mn2+-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea

Overview of attention for article published in Fungal Genetics & Biology, February 2015
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Title
bcpmr1 encodes a P-type Ca2+/Mn2+-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea
Published in
Fungal Genetics & Biology, February 2015
DOI 10.1016/j.fgb.2015.01.012
Pubmed ID
Authors

Verónica Plaza, Yanssuy Lagües, Mauro Carvajal, Luis A. Pérez-García, Hector M. Mora-Montes, Paulo Canessa, Luis F. Larrondo, Luis Castillo

Abstract

The cell wall of fungi is generally composed of an inner skeletal layer consisting of various polysaccharides surrounded by a layer of glycoproteins. These usually contain both N- and O-linked oligosaccharides, coupled to the proteins by stepwise addition of mannose residues by mannosyltransferases in the endoplasmic reticulum and the Golgi apparatus. In yeast, an essential luminal cofactor for these mannosyltransferases is Mn(2+) provided by the Ca(2+)/Mn(2+)-ATPase known as Pmr1. In this study, we have identified and characterized the B. cinerea pmr1 gene, the closest homolog of yeast PMR1. We hypothesized that bcpmr1 also encodes a Ca(2+)/Mn(2+)-ATPase that plays an important role in the protein glycosylation pathway. Phenotypic analysis showed that bcpmr1 null mutants displayed a significant reduction in conidial production, radial growth and diameter of sclerotia. Significant alterations in hyphal cell wall composition were observed including a 83% decrease of mannan levels and an increase in the amount of chitin and glucan. These changes were accompanied by a hypersensitivity to cell wall-perturbing agents such as Calcofluor white, Congo red and zymolyase. Importantly, the Δbcpmr1 mutant showed reduced virulence in tomato (leafs and fruits) and apple (fruits) and reduced biofilm formation. Together, our results highlight the importance of bcpmr1 for protein glycosylation, cell wall structure and virulence of B. cinerea.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Malaysia 1 2%
Unknown 44 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 16%
Student > Ph. D. Student 7 16%
Researcher 5 11%
Student > Master 3 7%
Professor 3 7%
Other 4 9%
Unknown 16 36%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 33%
Biochemistry, Genetics and Molecular Biology 4 9%
Immunology and Microbiology 2 4%
Chemistry 2 4%
Engineering 2 4%
Other 3 7%
Unknown 17 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 November 2015.
All research outputs
#19,962,154
of 25,394,764 outputs
Outputs from Fungal Genetics & Biology
#838
of 1,115 outputs
Outputs of similar age
#257,353
of 364,905 outputs
Outputs of similar age from Fungal Genetics & Biology
#10
of 26 outputs
Altmetric has tracked 25,394,764 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,115 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 364,905 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 26 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.