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Mendeley readers
Attention Score in Context
| Title |
The Promoter of the pri-miR-375 Gene Directs Expression Selectively to the Endocrine Pancreas
|
|---|---|
| Published in |
PLOS ONE, April 2009
|
| DOI | 10.1371/journal.pone.0005033 |
| Pubmed ID | |
| Authors |
Tali Avnit-Sagi, Lia Kantorovich, Sharon Kredo-Russo, Eran Hornstein, Michael D. Walker |
| Abstract |
microRNAs (miRNAs) are known to play an essential role in controlling a broad range of biological processes including animal development. Accordingly, many miRNAs are expressed preferentially in one or a small number of cell types. Yet the mechanisms responsible for this selectivity are not well understood. The aim of this study was to elucidate the molecular basis of cell-specific expression of the pri-miR-375 gene, which is selectively expressed in pancreatic islets, and has been implicated both in the development of islets, and the function of mature pancreatic beta cells. An evolutionarily conserved 768 bp region of DNA upstream of the pri-miR-375 gene was linked to GFP and luciferase reporter genes, and expression monitored in transgenic mice and transfected cultured cells. Deletion and targeted mutagenesis analysis was used to evaluate the functional significance of sequence blocks within the upstream fragment. 5'-RACE analysis was used for mapping the pri-miR-375 gene transcription start site. The conserved 768 bp region was able to direct preferential expression of a GFP reporter gene to pancreatic islets in transgenic mice. Deletion analysis using a luciferase reporter gene in transfected cultured cell lines confirmed the cell specificity of the putative promoter region, and identified several key cis-elements essential for optimal activity, including E-boxes and a TATA sequence. Consistent with this, 5'-RACE analysis identified a transcription start site within this DNA region, 24 bp downstream of the TATA sequence. These studies define the promoter of the pri-miR-375 gene, and show that islet-specific expression of the pri-miR-375 gene is controlled at the transcriptional level. Detailed analysis of the transcriptional mechanisms controlling expression of miRNA genes will be essential to permit a comprehensive understanding of the complex role of miRNAs such as miR-375 in developmental processes. |
Mendeley readers
The data shown below were compiled from readership statistics for 113 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | <1% |
| United States | 1 | <1% |
| China | 1 | <1% |
| Austria | 1 | <1% |
| Unknown | 109 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 29 | 26% |
| Student > Ph. D. Student | 24 | 21% |
| Student > Master | 13 | 12% |
| Professor > Associate Professor | 8 | 7% |
| Student > Bachelor | 6 | 5% |
| Other | 15 | 13% |
| Unknown | 18 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 52 | 46% |
| Biochemistry, Genetics and Molecular Biology | 23 | 20% |
| Medicine and Dentistry | 10 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 2% |
| Immunology and Microbiology | 2 | 2% |
| Other | 4 | 4% |
| Unknown | 20 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 November 2010.
All research outputs
#7,455,523
of 22,792,160 outputs
Outputs from PLOS ONE
#88,772
of 194,548 outputs
Outputs of similar age
#32,901
of 93,465 outputs
Outputs of similar age from PLOS ONE
#265
of 500 outputs
Altmetric has tracked 22,792,160 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 194,548 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.1. This one is in the 49th percentile – i.e., 49% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 93,465 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 19th percentile – i.e., 19% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 500 others from the same source and published within six weeks on either side of this one. This one is in the 20th percentile – i.e., 20% of its contemporaries scored the same or lower than it.