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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Human Engineered Heart Tissue as a Versatile Tool in Basic Research and Preclinical Toxicology
|
|---|---|
| Published in |
PLOS ONE, October 2011
|
| DOI | 10.1371/journal.pone.0026397 |
| Pubmed ID | |
| Authors |
Sebastian Schaaf, Aya Shibamiya, Marco Mewe, Alexandra Eder, Andrea Stöhr, Marc N. Hirt, Thomas Rau, Wolfram-Hubertus Zimmermann, Lenard Conradi, Thomas Eschenhagen, Arne Hansen |
| Abstract |
Human embryonic stem cell (hESC) progenies hold great promise as surrogates for human primary cells, particularly if the latter are not available as in the case of cardiomyocytes. However, high content experimental platforms are lacking that allow the function of hESC-derived cardiomyocytes to be studied under relatively physiological and standardized conditions. Here we describe a simple and robust protocol for the generation of fibrin-based human engineered heart tissue (hEHT) in a 24-well format using an unselected population of differentiated human embryonic stem cells containing 30-40% α-actinin-positive cardiac myocytes. Human EHTs started to show coherent contractions 5-10 days after casting, reached regular (mean 0.5 Hz) and strong (mean 100 µN) contractions for up to 8 weeks. They displayed a dense network of longitudinally oriented, interconnected and cross-striated cardiomyocytes. Spontaneous hEHT contractions were analyzed by automated video-optical recording and showed chronotropic responses to calcium and the β-adrenergic agonist isoprenaline. The proarrhythmic compounds E-4031, quinidine, procainamide, cisapride, and sertindole exerted robust, concentration-dependent and reversible decreases in relaxation velocity and irregular beating at concentrations that recapitulate findings in hERG channel assays. In conclusion this study establishes hEHT as a simple in vitro model for heart research. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 409 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 4 | <1% |
| Germany | 1 | <1% |
| Switzerland | 1 | <1% |
| Hungary | 1 | <1% |
| France | 1 | <1% |
| Italy | 1 | <1% |
| United Kingdom | 1 | <1% |
| Indonesia | 1 | <1% |
| Belgium | 1 | <1% |
| Other | 3 | <1% |
| Unknown | 394 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 115 | 28% |
| Researcher | 60 | 15% |
| Student > Master | 56 | 14% |
| Student > Bachelor | 40 | 10% |
| Student > Doctoral Student | 35 | 9% |
| Other | 50 | 12% |
| Unknown | 53 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 101 | 25% |
| Medicine and Dentistry | 69 | 17% |
| Engineering | 68 | 17% |
| Biochemistry, Genetics and Molecular Biology | 57 | 14% |
| Pharmacology, Toxicology and Pharmaceutical Science | 11 | 3% |
| Other | 36 | 9% |
| Unknown | 67 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 March 2024.
All research outputs
#2,378,004
of 23,515,383 outputs
Outputs from PLOS ONE
#30,063
of 201,473 outputs
Outputs of similar age
#12,342
of 141,049 outputs
Outputs of similar age from PLOS ONE
#330
of 2,569 outputs
Altmetric has tracked 23,515,383 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 201,473 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.3. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 141,049 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 2,569 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.