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Epigenetics Protocols

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Cover of 'Epigenetics Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Advances in epigenetic technology.
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    Chapter 2 DNA methylation detection: Bisulfite genomic sequencing analysis
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    Chapter 3 Methylation-Specific PCR
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    Chapter 4 Analysing DNA methylation using bisulphite pyrosequencing.
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    Chapter 5 Closed-Tube PCR Methods for Locus-Specific DNA Methylation Analysis
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    Chapter 6 A Combined Bisulfite Restriction Analysis Bioinformatics Tool: Methyl-Typing
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    Chapter 7 SIRPH: An HPLC-Based SNuPE for Quantitative Methylation Measurement at Specific CpG Sites
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    Chapter 8 Restriction Landmark Genome Scanning
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    Chapter 9 Methylated DNA Immunoprecipitation Genome-Wide Analysis
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    Chapter 10 Methylated-CpG Island Recovery Assay.
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    Chapter 11 Global DNA Methylation Analysis Using the Luminometric Methylation Assay
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    Chapter 12 Inhibition of DNA methylation in somatic cells.
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    Chapter 13 DNA Methyltransferase Assays
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    Chapter 14 A Chromatin Immunoprecipitation Protocol for Small Cell Numbers
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    Chapter 15 Native Chromatin Immunoprecipitation
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    Chapter 16 Q-PCR in Combination with ChIP Assays to Detect Changes in Chromatin Acetylation
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    Chapter 17 Sequential Chromatin Immunoprecipitation Assay and Analysis
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    Chapter 18 Combined chromatin immunoprecipitation and bisulfite methylation sequencing analysis.
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    Chapter 19 Studying RNA–Protein Interactions In Vivo By RNA Immunoprecipitation
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    Chapter 20 Using ChIP-Seq Technology to Generate High-Resolution Profiles of Histone Modifications
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    Chapter 21 Mapping Open Chromatin with Formaldehyde-Assisted Isolation of Regulatory Elements
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    Chapter 22 Inhibition of histone deacetylases.
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    Chapter 23 Computational Methods for Epigenetic Analysis: The Protocol of Computational Analysis for Modified Methylation-Specific Digital Karyotyping Based on Massively Parallel Sequencing
Attention for Chapter 1: Advances in epigenetic technology.
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Chapter title
Advances in epigenetic technology.
Chapter number 1
Book title
Epigenetics Protocols
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-316-5_1
Pubmed ID
Book ISBNs
978-1-61779-315-8, 978-1-61779-316-5
Authors

Tollefsbol TO, Trygve O. Tollefsbol, Tollefsbol, Trygve O.

Abstract

Epigenetics refers to the collective heritable changes in phenotype that arise independent of genotype. Two broad areas of epigenetics are DNA methylation and histone modifications and numerous techniques have been invented to analyze epigenetic processes not only at the level of specific genes, but also to analyze epigenetic changes that occur in defined regions of the genome as well as genome-wide. Advances have also been made in techniques devised to assess the enzymes that mediate epigenetic processes. These methods that are currently driving the field of epigenetics will greatly facilitate continued expansion of this exponentially growing discipline of genetics.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Korea, Republic of 1 2%
Unknown 57 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 28%
Student > Master 13 22%
Researcher 7 12%
Student > Bachelor 7 12%
Student > Doctoral Student 3 5%
Other 3 5%
Unknown 9 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 38%
Biochemistry, Genetics and Molecular Biology 7 12%
Medicine and Dentistry 5 9%
Neuroscience 3 5%
Pharmacology, Toxicology and Pharmaceutical Science 2 3%
Other 7 12%
Unknown 12 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 September 2011.
All research outputs
#15,234,609
of 22,651,245 outputs
Outputs from Methods in molecular biology
#5,279
of 13,012 outputs
Outputs of similar age
#140,016
of 180,239 outputs
Outputs of similar age from Methods in molecular biology
#141
of 229 outputs
Altmetric has tracked 22,651,245 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,012 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
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We're also able to compare this research output to 229 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.