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Dependence of Selective Gene Activation on the Androgen Receptor NH2- and COOH-terminal Interaction

Overview of attention for article published in Journal of Biological Chemistry, July 2002
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Dependence of Selective Gene Activation on the Androgen Receptor NH2- and COOH-terminal Interaction
Published in
Journal of Biological Chemistry, July 2002
DOI 10.1074/jbc.m202809200
Pubmed ID

Bin He, Lori W. Lee, John T. Minges, Elizabeth M. Wilson


The agonist-induced androgen receptor NH(2)- and COOH-terminal (N/C) interaction is mediated by the FXXLF and WXXLF NH(2)-terminal motifs. Here we demonstrate that agonist-dependent transactivation of prostate-specific antigen (PSA) and probasin enhancer/promoter regions requires the N/C interaction, whereas the sex-limited protein gene and mouse mammary tumor virus long terminal repeat do not. Transactivation of PSA and probasin response regions also depends on activation function 1 (AF1) in the NH(2)-terminal region but can be increased by binding an overexpressed p160 coactivator to activation function 2 (AF2) in the ligand binding domain. The dependence of the PSA and probasin enhancer/promoters on the N/C interaction for transactivation allowed us to demonstrate that in the presence of androgen, the WXXLF motif with the sequence (433)WHTLF(437) contributes as an inhibitor to AR transactivation. We further show that like the FXXLF and LXXLL motifs, the WXXLF motif interacts in the presence of androgen with AF2 in the ligand binding domain. Sequence comparisons among species indicate greater conservation of the FXXLF motif compared with the WXXLF motif, paralleling the functional significance of these binding motifs. The data provide evidence for promoter-specific differences in the requirement for the androgen receptor N/C interaction and in the contributions of AF1 and AF2 in androgen-induced gene regulation.

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 43 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 16 37%
Researcher 7 16%
Student > Master 5 12%
Student > Doctoral Student 2 5%
Lecturer 2 5%
Other 9 21%
Unknown 2 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 19 44%
Biochemistry, Genetics and Molecular Biology 9 21%
Medicine and Dentistry 4 9%
Chemistry 3 7%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Other 0 0%
Unknown 7 16%