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Genetic characterization of a novel picorna-like virus in Culex spp. mosquitoes from Mozambique

Overview of attention for article published in Virology Journal, April 2018
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  • Above-average Attention Score compared to outputs of the same age (58th percentile)

Mentioned by

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6 tweeters

Citations

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5 Dimensions

Readers on

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19 Mendeley
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Title
Genetic characterization of a novel picorna-like virus in Culex spp. mosquitoes from Mozambique
Published in
Virology Journal, April 2018
DOI 10.1186/s12985-018-0981-z
Pubmed ID
Authors

Harindranath Cholleti, Juliette Hayer, Jose Fafetine, Mikael Berg, Anne-Lie Blomström

Abstract

Mosquitoes are the potential vectors for a variety of viruses that can cause diseases in the human and animal populations. Viruses in the order Picornavirales infect a broad range of hosts, including mosquitoes. In this study, we aimed to characterize a novel picorna-like virus from the Culex spp. of mosquitoes from the Zambezi Valley of Mozambique. The extracted RNA from mosquito pools was pre-amplified with the sequence independent single primer amplification (SISPA) method and subjected to high-throughput sequencing using the Ion Torrent platform. Reads that are classified as Iflaviridae, Picornaviridae and Dicistroviridae were assembled by CodonCode Aligner and SPAdes. Gaps between the viral contigs were sequenced by PCR. The genomic ends were analyzed by 5' and 3' RACE PCRs. The ORF was predicted with the NCBI ORF finder. The conserved domains were identified with ClustalW multiple sequence alignment, and a phylogenetic tree was built with MEGA. The presence of the virus in individual mosquito pools was detected by RT-PCR assay. A near full-length viral genome (9740 nt) was obtained in Culex mosquitoes that encoded a complete ORF (3112 aa), named Culex picorna-like virus (CuPV-1). The predicted ORF had 38% similarity to the Hubei picorna-like virus 35. The sequence of the conserved domains, Helicase-Protease-RNA-dependent RNA polymerase, were identified by multiple sequence alignment and found to be at the 3' end, similar to iflaviruses. Phylogenetic analysis of the putative RdRP amino acid sequences indicated that the virus clustered with members of the Iflaviridae family. CuPV-1 was detected in both Culex and Mansonia individual pools with low infection rates. The study reported a highly divergent, near full-length picorna-like virus genome from Culex spp. mosquitoes from Mozambique. The discovery and characterization of novel viruses in mosquitoes is an initial step, which will provide insights into mosquito-virus interaction mechanisms, genetic diversity and evolution.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 32%
Student > Master 4 21%
Student > Bachelor 3 16%
Student > Ph. D. Student 1 5%
Other 1 5%
Other 2 11%
Unknown 2 11%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 21%
Biochemistry, Genetics and Molecular Biology 3 16%
Arts and Humanities 2 11%
Immunology and Microbiology 2 11%
Environmental Science 1 5%
Other 3 16%
Unknown 4 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 November 2018.
All research outputs
#7,195,953
of 14,218,657 outputs
Outputs from Virology Journal
#751
of 2,258 outputs
Outputs of similar age
#112,135
of 276,512 outputs
Outputs of similar age from Virology Journal
#1
of 1 outputs
Altmetric has tracked 14,218,657 research outputs across all sources so far. This one is in the 49th percentile – i.e., 49% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,258 research outputs from this source. They receive a mean Attention Score of 3.5. This one has gotten more attention than average, scoring higher than 65% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 276,512 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them