Title |
Methylation analysis of histone H4K12ac-associated promoters in sperm of healthy donors and subfertile patients
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Published in |
Clinical Epigenetics, March 2015
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DOI | 10.1186/s13148-015-0058-4 |
Pubmed ID | |
Authors |
Markus Vieweg, Katerina Dvorakova-Hortova, Barbora Dudkova, Przemyslaw Waliszewski, Marie Otte, Berthold Oels, Amir Hajimohammad, Heiko Turley, Martin Schorsch, Hans-Christian Schuppe, Wolfgang Weidner, Klaus Steger, Agnieszka Paradowska-Dogan |
Abstract |
Histone to protamine exchange and the hyperacetylation of the remaining histones are hallmarks of spermiogenesis. Acetylation of histone H4 at lysine 12 (H4K12ac) was observed prior to full decondensation of sperm chromatin after fertilization suggesting an important role for the regulation of gene expression in early embryogenesis. Similarly, DNA methylation may contribute to gene silencing of several developmentally important genes. Following the identification of H4K12ac-binding promoters in sperm of fertile and subfertile patients, we aimed to investigate whether the depletion of histone-binding is associated with aberrant DNA methylation in sperm of subfertile men. Furthermore, we monitored the transmission of H4K12ac, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) from the paternal chromatin to the embryo applying mouse in vitro fertilization and immunofluorescence. |
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Student > Bachelor | 8 | 14% |
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Student > Master | 7 | 12% |
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Earth and Planetary Sciences | 1 | 2% |
Other | 3 | 5% |
Unknown | 13 | 23% |