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Age-related changes in spleen of Dark Agouti rats immunized for experimental autoimmune encephalomyelitis

Overview of attention for article published in Journal of Neuroimmunology, January 2015
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Title
Age-related changes in spleen of Dark Agouti rats immunized for experimental autoimmune encephalomyelitis
Published in
Journal of Neuroimmunology, January 2015
DOI 10.1016/j.jneuroim.2014.12.014
Pubmed ID
Authors

Jasmina Djikić, Mirjana Nacka-Aleksić, Ivan Pilipović, Duško Kosec, Nevena Arsenović-Ranin, Zorica Stojić-Vukanić, Mirjana Dimitrijević, Gordana Leposavić

Abstract

The study was undertaken considering age-related changes in susceptibility to experimental autoimmune encephalomyelitis (EAE) and a putative role of spleen in pathogenesis of this disease. The phenotypic and functional characteristics of T splenocytes were examined in young (3-month-old), middle-aged (8-month-old) and aged (26-month-old) Dark Agouti rats immunized for EAE with rat spinal cord in complete Freund's adjuvant. The rat susceptibility to EAE induction, as well as the number of activated CD4+CD134+ lymphocytes retrieved from their spinal cords progressively decreased with aging. To the contrary, in rats immunized for EAE the number of activated CD4+ splenocytes, i.e., CD4+CD134+, CD4+CD25+FoxP3- and CD4+CD40L+ cells, progressively increased with aging. This was associated with age-related increase in (i) CD4+ splenocyte surface expression of CD44, the molecule suggested to be involved in limiting emigration of encephalitogenic CD4+ cells from spleen into blood and (ii) frequency of regulatory T cells, including CD4+CD25+FoxP3+ cells, which are also shown to control encephalitogenic cell migration from spleen into the central nervous system. In favor of expansion of T-regulatory cell pool in aged rats was the greater concentration of IL-10 in unstimulated, Concanavalin A (ConA)- and myelin basic protein (MBP)-stimulated splenocyte cultures from aged rats compared with the corresponding cultures from young ones. Consistent with the age-related increase in the expression of CD44, which is shown to favor Th1 effector cell survival by interfering with CD95-mediated signaling, the frequency of apoptotic cells among CD4+ splenocytes, despite the greater frequency of CD95+ cells, was diminished in splenocyte cultures from aged compared with young rats. In addition, in control, as well as in ConA- and MBP-stimulated splenocyte cultures from aged rats, despite of impaired CD4+ cell proliferation, IFN-γ concentrations were greater than in corresponding cultures from young rats. This most likely reflected increased abundance of IFN-γ-producing cells in splenocyte cultures from aged compared with young rats. The diminished CD4+ cell proliferation in response to ConA and MBP in splenocyte cultures from aged compared with young rats could be, at least partly, associated with an enhanced splenic expression of iNOS mRNA in aged rats. Thus, the study suggests that age-associated changes leading to entrapping of activated CD4+ cells in the spleen could contribute to the restriction in development of EAE in aged rats.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 6%
Unknown 15 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 19%
Student > Doctoral Student 2 13%
Student > Master 2 13%
Professor > Associate Professor 2 13%
Student > Bachelor 2 13%
Other 4 25%
Unknown 1 6%
Readers by discipline Count As %
Neuroscience 3 19%
Veterinary Science and Veterinary Medicine 2 13%
Biochemistry, Genetics and Molecular Biology 2 13%
Medicine and Dentistry 2 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Other 5 31%
Unknown 1 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 March 2015.
All research outputs
#10,887,703
of 12,284,947 outputs
Outputs from Journal of Neuroimmunology
#1,834
of 2,064 outputs
Outputs of similar age
#185,910
of 222,513 outputs
Outputs of similar age from Journal of Neuroimmunology
#20
of 24 outputs
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So far Altmetric has tracked 2,064 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.