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Targeted epigenome editing of an endogenous locus with chromatin modifiers is not stably maintained

Overview of attention for article published in Epigenetics & Chromatin, March 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (88th percentile)

Mentioned by

blogs
1 blog
twitter
10 tweeters

Citations

dimensions_citation
56 Dimensions

Readers on

mendeley
109 Mendeley
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Title
Targeted epigenome editing of an endogenous locus with chromatin modifiers is not stably maintained
Published in
Epigenetics & Chromatin, March 2015
DOI 10.1186/s13072-015-0002-z
Pubmed ID
Authors

Goran Kungulovski, Suneetha Nunna, Maria Thomas, Ulrich M Zanger, Richard Reinhardt, Albert Jeltsch

Abstract

DNA methylation and histone 3 lysine 9 (H3K9) methylation are considered as epigenetic marks that can be inherited through cell divisions. To explore the functional consequences and stability of these modifications, we employed targeted installment of DNA methylation and H3K9 methylation in the vascular endothelial growth factor A (VEGF-A) promoter using catalytic domains of DNA or H3K9 methyltransferases that are fused to a zinc finger protein which binds a site in the VEGF-A promoter. Expression of the targeted DNA and H3K9 methyltransferases caused dense deposition of DNA methylation or H3K9 di- and trimethylation in the promoter of VEGF-A and downregulation of VEGF-A gene expression. We did not observe positive feedback between DNA methylation and H3K9 methylation. Upon loss of the targeted methyltransferases from the cells, the epigenetic marks, chromatin environment, and gene expression levels returned to their original state, indicating that both methylation marks were not stably propagated after their installment. The clear anti-correlation between DNA or H3K9 methylation and gene expression suggests a direct role of these marks in transcriptional control. The lack of maintenance of the transiently induced silenced chromatin state suggests that the stability of epigenetic signaling is based on an epigenetic network consisting of several molecular marks. Therefore, for stable reprogramming, either multivalent deposition of functionally related epigenetic marks or longer-lasting trigger stimuli might be necessary.

Twitter Demographics

The data shown below were collected from the profiles of 10 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 109 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 2 2%
Netherlands 2 2%
United Kingdom 2 2%
Bulgaria 1 <1%
Germany 1 <1%
Spain 1 <1%
Unknown 100 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 33 30%
Student > Ph. D. Student 31 28%
Student > Master 14 13%
Professor > Associate Professor 9 8%
Student > Bachelor 6 6%
Other 11 10%
Unknown 5 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 56 51%
Biochemistry, Genetics and Molecular Biology 31 28%
Medicine and Dentistry 3 3%
Immunology and Microbiology 3 3%
Pharmacology, Toxicology and Pharmaceutical Science 2 2%
Other 6 6%
Unknown 8 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 July 2017.
All research outputs
#1,317,443
of 14,207,018 outputs
Outputs from Epigenetics & Chromatin
#56
of 428 outputs
Outputs of similar age
#26,838
of 225,648 outputs
Outputs of similar age from Epigenetics & Chromatin
#1
of 1 outputs
Altmetric has tracked 14,207,018 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 428 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 225,648 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them