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The genome of cultivated sweet potato containsAgrobacteriumT-DNAs with expressed genes: An example of a naturally transgenic food crop

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, April 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#43 of 47,635)
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

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mendeley
498 Mendeley
citeulike
2 CiteULike
Title
The genome of cultivated sweet potato containsAgrobacteriumT-DNAs with expressed genes: An example of a naturally transgenic food crop
Published in
Proceedings of the National Academy of Sciences of the United States of America, April 2015
DOI 10.1073/pnas.1419685112
Pubmed ID
Authors

Tina Kyndt, Dora Quispe, Hong Zhai, Robert Jarret, Marc Ghislain, Qingchang Liu, Godelieve Gheysen, Jan F. Kreuze, Kyndt T, Quispe D, Zhai H, Jarret R, Ghislain M, Liu Q, Gheysen G, Kreuze JF, Kyndt, T., Quispe, D., Zhai, H., Jarret, R., Ghislain, M., Liu, Q., Gheysen, G., Kreuze, J.F.

Abstract

Agrobacterium rhizogenes and Agrobacterium tumefaciens are plant pathogenic bacteria capable of transferring DNA fragments [transfer DNA (T-DNA)] bearing functional genes into the host plant genome. This naturally occurring mechanism has been adapted by plant biotechnologists to develop genetically modified crops that today are grown on more than 10% of the world's arable land, although their use can result in considerable controversy. While assembling small interfering RNAs, or siRNAs, of sweet potato plants for metagenomic analysis, sequences homologous to T-DNA sequences from Agrobacterium spp. were discovered. Simple and quantitative PCR, Southern blotting, genome walking, and bacterial artificial chromosome library screening and sequencing unambiguously demonstrated that two different T-DNA regions (IbT-DNA1 and IbT-DNA2) are present in the cultivated sweet potato (Ipomoea batatas [L.] Lam.) genome and that these foreign genes are expressed at detectable levels in different tissues of the sweet potato plant. IbT-DNA1 was found to contain four open reading frames (ORFs) homologous to the tryptophan-2-monooxygenase (iaaM), indole-3-acetamide hydrolase (iaaH), C-protein (C-prot), and agrocinopine synthase (Acs) genes of Agrobacterium spp. IbT-DNA1 was detected in all 291 cultigens examined, but not in close wild relatives. IbT-DNA2 contained at least five ORFs with significant homology to the ORF14, ORF17n, rooting locus (Rol)B/RolC, ORF13, and ORF18/ORF17n genes of A. rhizogenes. IbT-DNA2 was detected in 45 of 217 genotypes that included both cultivated and wild species. Our finding, that sweet potato is naturally transgenic while being a widely and traditionally consumed food crop, could affect the current consumer distrust of the safety of transgenic food crops.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 498 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 13 3%
Germany 7 1%
Japan 5 1%
Brazil 4 <1%
Spain 4 <1%
Mexico 3 <1%
Italy 3 <1%
Switzerland 3 <1%
Australia 2 <1%
Other 18 4%
Unknown 436 88%

Demographic breakdown

Readers by professional status Count As %
Researcher 120 24%
Student > Ph. D. Student 115 23%
Student > Master 69 14%
Student > Bachelor 57 11%
Other 32 6%
Other 105 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 382 77%
Biochemistry, Genetics and Molecular Biology 56 11%
Unspecified 17 3%
Chemistry 6 1%
Environmental Science 6 1%
Other 31 6%

Attention Score in Context

This research output has an Altmetric Attention Score of 1238. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 November 2017.
All research outputs
#1,201
of 8,658,076 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#43
of 47,635 outputs
Outputs of similar age
#42
of 207,401 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#2
of 1,015 outputs
Altmetric has tracked 8,658,076 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 47,635 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 25.0. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 207,401 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 1,015 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.