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CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes

Overview of attention for article published in Protein & Cell, April 2015
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#1 of 299)
  • High Attention Score compared to outputs of the same age (99th percentile)
  • High Attention Score compared to outputs of the same age and source (90th percentile)

Readers on

mendeley
1414 Mendeley
citeulike
6 CiteULike
Title
CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes
Published in
Protein & Cell, April 2015
DOI 10.1007/s13238-015-0153-5
Pubmed ID
Authors

Puping Liang, Yanwen Xu, Xiya Zhang, Chenhui Ding, Rui Huang, Zhen Zhang, Jie Lv, Xiaowei Xie, Yuxi Chen, Yujing Li, Ying Sun, Yaofu Bai, Zhou Songyang, Wenbin Ma, Canquan Zhou, Junjiu Huang, Liang, Puping, Xu, Yanwen, Zhang, Xiya, Ding, Chenhui, Huang, Rui, Zhang, Zhen, Lv, Jie, Xie, Xiaowei, Chen, Yuxi, Li, Yujing, Sun, Ying, Bai, Yaofu, Songyang, Zhou, Ma, Wenbin, Zhou, Canquan, Huang, Junjiu

Abstract

Genome editing tools such as the clustered regularly interspaced short palindromic repeat (CRISPR)-associated system (Cas) have been widely used to modify genes in model systems including animal zygotes and human cells, and hold tremendous promise for both basic research and clinical applications. To date, a serious knowledge gap remains in our understanding of DNA repair mechanisms in human early embryos, and in the efficiency and potential off-target effects of using technologies such as CRISPR/Cas9 in human pre-implantation embryos. In this report, we used tripronuclear (3PN) zygotes to further investigate CRISPR/Cas9-mediated gene editing in human cells. We found that CRISPR/Cas9 could effectively cleave the endogenous β-globin gene (HBB). However, the efficiency of homologous recombination directed repair (HDR) of HBB was low and the edited embryos were mosaic. Off-target cleavage was also apparent in these 3PN zygotes as revealed by the T7E1 assay and whole-exome sequencing. Furthermore, the endogenous delta-globin gene (HBD), which is homologous to HBB, competed with exogenous donor oligos to act as the repair template, leading to untoward mutations. Our data also indicated that repair of the HBB locus in these embryos occurred preferentially through the non-crossover HDR pathway. Taken together, our work highlights the pressing need to further improve the fidelity and specificity of the CRISPR/Cas9 platform, a prerequisite for any clinical applications of CRSIPR/Cas9-mediated editing.

Twitter Demographics

The data shown below were collected from the profiles of 446 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 1,414 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 29 2%
United Kingdom 11 <1%
Germany 6 <1%
Japan 6 <1%
France 4 <1%
Brazil 4 <1%
China 3 <1%
Russian Federation 3 <1%
Italy 2 <1%
Other 29 2%
Unknown 1317 93%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 401 28%
Student > Ph. D. Student 276 20%
Student > Master 261 18%
Researcher 210 15%
Other 60 4%
Other 206 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 688 49%
Biochemistry, Genetics and Molecular Biology 328 23%
Medicine and Dentistry 133 9%
Engineering 44 3%
Unspecified 41 3%
Other 180 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1969. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 November 2017.
All research outputs
#369
of 8,642,801 outputs
Outputs from Protein & Cell
#1
of 299 outputs
Outputs of similar age
#15
of 207,620 outputs
Outputs of similar age from Protein & Cell
#1
of 10 outputs
Altmetric has tracked 8,642,801 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 299 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.9. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 207,620 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them