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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Antibacterial, antibiofilm and cytotoxic activities of Terminalia fagifolia Mart. extract and fractions
|
|---|---|
| Published in |
Annals of Clinical Microbiology and Antimicrobials, April 2015
|
| DOI | 10.1186/s12941-015-0084-2 |
| Pubmed ID | |
| Authors |
Alyne Rodrigues de Araujo, Patrick Veras Quelemes, Márcia Luana Gomes Perfeito, Luíza Ianny de Lima, Melka Coêlho Sá, Paulo Humberto Moreira Nunes, Graziella Anselmo Joanitti, Peter Eaton, Maria José dos Santos Soares, José Roberto de Souza de Almeida Leite |
| Abstract |
The methicillin resistance of bacteria from the genus Staphylococcus and its ability to form biofilms are important factors in pathogenesis of these microorganisms. Thus, the search for new antimicrobials agents, especially from plants, has been intensified. In this context, Terminalia species have been the subject of research for many pharmacological activities. In this study we evaluated the antibacterial, antibiofilm and cytotoxic activities of the ethanol extract (EtE) from Terminalia fagifolia stem bark as well as that of three fractions of the extract (AqF, HaF and WSF). We determined the minimum inhibitory concentration (MIC) by microdilution in 96-well plates, where the strains were exposed to serial dilutions of the ethanol extract and fractions, ranging from 12.5 to 400 μg/mL. We then determined the minimum bactericidal concentration (MBC), seeding the inoculum (10 μL) with concentrations equal to or greater than the MIC in Mueller-Hinton agar. To test the antibiofilm activity biofilm formation was induced in the presence of concentrations equivalent to 1/2, 1/4 and 1/8 of the MIC extract or fraction tested. In addition, the effect of the EtE and the fractions on cell viability was tested by the MTT assay on human MCF-7 breast cancer and mouse fibroblast NIH/3T3. To obtain high-resolution images of the effect of the aqueous fraction on the bacterial morphology, atomic force microscopy (AFM) imaging of treated S. aureus cells was performed. We observed antibacterial activity of EtE and fractions with MICs ranging from 25-200 μg/mL and MBCs ranging from 200-400 μg/mL. Regarding antibiofilm activity, both the EtE as the AqF, HaF and WSF fractions showed significant inhibition of the biofilm formation, with inhibition of biofilms formation of over 80% for some strains. The EtE and fractions showed a moderate cytotoxicity in cell line NIH/3T3 viability and potential antitumoral activity on human breast cancer cell line MCF-7. The microscopic images obtained revealed morphological changes to the S. aureus ATCC 29213 surface caused by AqF, as well as significant size alterations. The results show potential antibacterial, antibiofilm and antitumoral activities of the ethanol extract and fractions of T. fagifolia. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Venezuela, Bolivarian Republic of | 1 | 33% |
| United Kingdom | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 67% |
| Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 84 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 1% |
| Unknown | 83 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 13 | 15% |
| Student > Bachelor | 13 | 15% |
| Student > Ph. D. Student | 10 | 12% |
| Researcher | 9 | 11% |
| Student > Postgraduate | 5 | 6% |
| Other | 9 | 11% |
| Unknown | 25 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 18% |
| Medicine and Dentistry | 10 | 12% |
| Biochemistry, Genetics and Molecular Biology | 9 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 7 | 8% |
| Immunology and Microbiology | 7 | 8% |
| Other | 9 | 11% |
| Unknown | 27 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 March 2016.
All research outputs
#14,808,845
of 22,800,560 outputs
Outputs from Annals of Clinical Microbiology and Antimicrobials
#314
of 607 outputs
Outputs of similar age
#148,627
of 265,270 outputs
Outputs of similar age from Annals of Clinical Microbiology and Antimicrobials
#7
of 15 outputs
Altmetric has tracked 22,800,560 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 607 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,270 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 15 others from the same source and published within six weeks on either side of this one. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.