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NOS2 deficiency has no influence on the radiosensitivity of the hematopoietic system

Overview of attention for article published in Cell & Bioscience, April 2018
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Title
NOS2 deficiency has no influence on the radiosensitivity of the hematopoietic system
Published in
Cell & Bioscience, April 2018
DOI 10.1186/s13578-018-0228-0
Pubmed ID
Authors

Chengcheng Li, Yi Luo, Lijian Shao, Aimin Meng, Daohong Zhou

Abstract

Previous studies have shown that inhibition of inducible NO synthase (NOS2 or iNOS) with an inhibitor can selectively protect several normal tissues against radiation during radiotherapy. However, the role of NOS2 in ionizing radiation (IR)-induced bone marrow (BM) suppression is unknown and thus was investigated in the present study using NOS2-/- and wild-type mice 14 days after they were exposed to a sublethal dose of total body irradiation (TBI). The effects of different doses of IR (1, 2 and 4 Gy) on the apoptosis and colony-forming ability of bone marrow cells from wild-type (WT) and NOS2-/- mice were investigated in vitro. In addition, we exposed NOS2-/- mice and WT mice to 6-Gy TBI or sham irradiation. They were euthanized 14 days after TBI for analysis of peripheral blood cell counts and bone marrow cellularity. Colony-forming unit-granulocyte and macrophage, burst-forming unit-erythroid and CFU-granulocyte, erythroid, macrophage in bone marrow cells from the mice were determined to evaluate the function of hematopoietic progenitor cells (HPCs), and the ability of hematopoietic stem cells (HSCs) to self-renew was analysed by the cobblestone area forming cell assay. The cell cycling of HPCs and HSCs were measured by flow cytometry. Exposure to 2 and 4 Gy IR induced bone marrow cell apoptosis and inhibited the proliferation of HPCs in vitro. However, there was no difference between the cells from WT mice and NOS2-/- mice in response to IR exposure in vitro. Exposure of WT mice and NOS2-/- mice to 6 Gy TBI decreased the white blood cell, red blood cell, and platelet counts in the peripheral blood and bone marrow mononuclear cells, and reduced the colony-forming ability of HPCs (P < 0.05), damaged the clonogenic function of HSCs. However, these changes were not significantly different in WT and NOS2-/- mice. These data suggest that IR induces BM suppression in a NOS2-independent manner.

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Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 33%
Librarian 1 33%
Student > Master 1 33%
Readers by discipline Count As %
Unspecified 1 33%
Pharmacology, Toxicology and Pharmaceutical Science 1 33%
Medicine and Dentistry 1 33%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 April 2018.
All research outputs
#10,266,682
of 12,861,409 outputs
Outputs from Cell & Bioscience
#174
of 289 outputs
Outputs of similar age
#203,707
of 270,039 outputs
Outputs of similar age from Cell & Bioscience
#2
of 6 outputs
Altmetric has tracked 12,861,409 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 289 research outputs from this source. They receive a mean Attention Score of 2.2. This one is in the 12th percentile – i.e., 12% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 270,039 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.