| Chapter title |
Identification of Lysine-Acetylated Mitochondrial Proteins and Their Acetylation Sites
|
|---|---|
| Chapter number | 7 |
| Book title |
Plant Mitochondria
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2639-8_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2638-1, 978-1-4939-2639-8
|
| Authors |
Markus Hartl, Ann-Christine König, Iris Finkemeier |
| Editors |
James Whelan, Monika W. Murcha |
| Abstract |
The (ε)N-acetylation of lysine side chains is a highly conserved posttranslational modification of both prokaryotic and eukaryotic proteins. Lysine acetylation not only occurs on histones in the nucleus but also on many mitochondrial proteins in plants and animals. As the transfer of the acetyl group to lysine eliminates its positive charge, lysine acetylation can affect the biological function of proteins. This chapter describes two methods for the identification of lysine-acetylated proteins in plant mitochondria using an anti-acetyllysine antibody. We describe the Western blot analysis of a two-dimensional blue native-polyacrylamide gel electrophoresis with an anti-acetyllysine antibody as well as the immuno-enrichment of lysine-acetylated peptides followed by liquid chromatography-tandem mass spectrometry data acquisition and analysis. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Austria | 1 | 9% |
| Unknown | 10 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 18% |
| Researcher | 2 | 18% |
| Lecturer > Senior Lecturer | 1 | 9% |
| Other | 1 | 9% |
| Professor | 1 | 9% |
| Other | 3 | 27% |
| Unknown | 1 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 45% |
| Agricultural and Biological Sciences | 3 | 27% |
| Unspecified | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Unknown | 1 | 9% |