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Structure-Function Analyses of the Interactions between Rab11 and Rab14 Small GTPases with Their Shared Effector Rab Coupling Protein (RCP)*

Overview of attention for article published in Journal of Biological Chemistry, May 2015
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Title
Structure-Function Analyses of the Interactions between Rab11 and Rab14 Small GTPases with Their Shared Effector Rab Coupling Protein (RCP)*
Published in
Journal of Biological Chemistry, May 2015
DOI 10.1074/jbc.m114.612366
Pubmed ID
Authors

Patrick Lall, Andrew J Lindsay, Sara Hanscom, Tea Kecman, Elizabeth S Taglauer, Una M McVeigh, Edward Franklin, Mary W McCaffrey, Amir R Khan

Abstract

Rab GTPases recruit effector proteins, via their GTP-dependent switch regions, to distinct sub-cellular compartments. Rab11 and Rab25 are closely related small GTPases that bind to common effectors termed the Rab11-Family of Interacting Proteins (FIPs). The FIPs are organized into two subclasses (class I and class II) based on sequence and domain organization, and both subclasses contain a highly conserved Rab-binding domain (RBD) at their C-termini. Yeast two-hybrid and biochemical studies have revealed that the more distantly related Rab14 also interacts with class I FIPs. Here, we perform detailed structural, thermodynamic and cellular analyses of the interactions between Rab14 and one of the class I FIPs - the Rab Coupling Protein (RCP) - to clarify the molecular aspects of the interaction. We find that Rab14 indeed binds to RCP, albeit with reduced affinity relative to conventional Rab11:FIP and Rab25:FIP complexes. However, in vivo, Rab11 recruits RCP onto biological membranes. Furthermore, biophysical analyses reveal a non-canonical 1:2 stoichiometry between Rab14:RCP in dilute solutions, in contrast to Rab11/25 complexes. The structure of Rab14:RCP reveals that Rab14 interacts with the canonical RBD, and also provides insight into the unusual properties of the complex. Finally, we show that both the Rab Coupling Protein and Rab14 function in neuritogenesis.

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Mendeley readers

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The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 2%
Germany 1 2%
Unknown 40 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 24%
Researcher 7 17%
Student > Bachelor 6 14%
Professor > Associate Professor 3 7%
Student > Master 2 5%
Other 5 12%
Unknown 9 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 33%
Agricultural and Biological Sciences 13 31%
Immunology and Microbiology 2 5%
Chemistry 2 5%
Neuroscience 1 2%
Other 0 0%
Unknown 10 24%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 June 2015.
All research outputs
#22,758,309
of 25,371,288 outputs
Outputs from Journal of Biological Chemistry
#82,452
of 85,238 outputs
Outputs of similar age
#240,003
of 281,284 outputs
Outputs of similar age from Journal of Biological Chemistry
#390
of 462 outputs
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So far Altmetric has tracked 85,238 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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