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Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries

Overview of attention for article published in Malaria Journal, June 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (76th percentile)
  • Good Attention Score compared to outputs of the same age and source (68th percentile)

Mentioned by

twitter
12 tweeters
facebook
1 Facebook page

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
35 Mendeley
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Title
Development of a genetic tool for functional screening of anti-malarial bioactive extracts in metagenomic libraries
Published in
Malaria Journal, June 2015
DOI 10.1186/s12936-015-0748-6
Pubmed ID
Authors

Alvaro Mongui, Francy J. Pérez-Llanos, Marcio M. Yamamoto, Marcela Lozano, Maria M. Zambrano, Patricia Del Portillo, Carmen Fernández-Becerra, Silvia Restrepo, Hernando A. Del Portillo, Howard Junca

Abstract

The chemical treatment of Plasmodium falciparum for human infections is losing efficacy each year due to the rise of resistance. One possible strategy to find novel anti-malarial drugs is to access the largest reservoir of genomic biodiversity source on earth present in metagenomes of environmental microbial communities. A bioluminescent P. falciparum parasite was used to quickly detect shifts in viability of microcultures grown in 96-well plates. A synthetic gene encoding the Dermaseptin 4 peptide was designed and cloned under tight transcriptional control in a large metagenomic insert context (30 kb) to serve as proof-of-principle for the screening platform. Decrease in parasite viability consistently correlated with bioluminescence emitted from parasite microcultures, after their exposure to bacterial extracts containing a plasmid or fosmid engineered to encode the Dermaseptin 4 anti-malarial peptide. Here, a new technical platform to access the anti-malarial potential in microbial environmental metagenomes has been developed.

Twitter Demographics

The data shown below were collected from the profiles of 12 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 3%
United States 1 3%
Unknown 33 94%

Demographic breakdown

Readers by professional status Count As %
Student > Master 8 23%
Researcher 6 17%
Student > Ph. D. Student 5 14%
Student > Doctoral Student 5 14%
Student > Bachelor 3 9%
Other 7 20%
Unknown 1 3%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 46%
Biochemistry, Genetics and Molecular Biology 5 14%
Medicine and Dentistry 3 9%
Environmental Science 2 6%
Immunology and Microbiology 2 6%
Other 4 11%
Unknown 3 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2016.
All research outputs
#1,932,936
of 9,727,899 outputs
Outputs from Malaria Journal
#675
of 3,283 outputs
Outputs of similar age
#52,036
of 226,761 outputs
Outputs of similar age from Malaria Journal
#34
of 109 outputs
Altmetric has tracked 9,727,899 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,283 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.2. This one has done well, scoring higher than 79% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 226,761 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 109 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.