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A lupane-triterpene isolated from Combretum leprosum Mart. fruit extracts that interferes with the intracellular development of Leishmania (L.) amazonensis in vitro

Overview of attention for article published in BMC Complementary and Alternative Medicine, June 2015
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4 tweeters

Citations

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19 Dimensions

Readers on

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39 Mendeley
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Title
A lupane-triterpene isolated from Combretum leprosum Mart. fruit extracts that interferes with the intracellular development of Leishmania (L.) amazonensis in vitro
Published in
BMC Complementary and Alternative Medicine, June 2015
DOI 10.1186/s12906-015-0681-9
Pubmed ID
Authors

Carolina Bioni Garcia Teles, Leandro Soares Moreira-Dill, Alexandre de Almeida Silva, Valdir Alves Facundo, Walter F. de Azevedo, Luiz Hildebrando Pereira da Silva, Maria Cristina M. Motta, Rodrigo Guerino Stábeli, Izaltina Silva-Jardim

Abstract

3beta,6beta,16beta-trihydroxylup-20(29)-ene is a lupane triterpene isolated from Combretum leprosum fruit. The lupane group has been extensively used in studies on anticancer effects; however, its possible activity against protozoa parasites is yet poorly known. The high toxicity of the compounds currently used in leishmaniasis chemotherapy stimulates the investigation of new molecules and drug targets for antileishmanial therapy. The activity of 3beta,6beta,16beta-trihydroxylup-20(29)-ene was evaluated against Leishmania (L.) amazonensis by determining the cytotoxicity of the compound on murine peritoneal macrophages, as well as its effects on parasite survival inside host cells. To evaluate the effect of this compound on intracellular amastigotes, cultures of infected macrophages were treated for 24, 48 and 96 h and the percentage of infected macrophages and the number of intracellular parasites was scored using light microscopy. Lupane showed significant activity against the intracellular amastigotes of L. (L.) amazonensis. The treatment with 109 μM for 96 h reduced in 80 % the survival index of parasites in BALB/c peritoneal macrophages. At this concentration, the triterpene caused no cytotoxic effects against mouse peritoneal macrophages. Ultrastructural analyses of L. (L.) amazonensis intracellular amastigotes showed that lupane induced some morphological changes in parasites, such as cytosolic vacuolization, lipid body formation and mitochondrial swelling. Bioinformatic analyses through molecular docking suggest that this lupane has high-affinity binding with DNA topoisomerase. Taken together, our results have showed that the lupane triterpene from C. leprosum interferes with L. (L.) amazonensis amastigote replication and survival inside vertebrate host cells and bioinformatics analyses strongly indicate that this molecule may be a potential inhibitor of topoisomerase IB. Moreover, this study opens major prospects for the development of novel chemotherapeutic agents with leishmanicidal activity.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 3%
Portugal 1 3%
Brazil 1 3%
Unknown 36 92%

Demographic breakdown

Readers by professional status Count As %
Student > Master 9 23%
Professor > Associate Professor 4 10%
Professor 4 10%
Student > Ph. D. Student 4 10%
Researcher 3 8%
Other 8 21%
Unknown 7 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 18%
Chemistry 6 15%
Agricultural and Biological Sciences 5 13%
Pharmacology, Toxicology and Pharmaceutical Science 3 8%
Immunology and Microbiology 3 8%
Other 3 8%
Unknown 12 31%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 November 2015.
All research outputs
#2,899,570
of 6,535,212 outputs
Outputs from BMC Complementary and Alternative Medicine
#883
of 1,732 outputs
Outputs of similar age
#96,715
of 187,979 outputs
Outputs of similar age from BMC Complementary and Alternative Medicine
#41
of 83 outputs
Altmetric has tracked 6,535,212 research outputs across all sources so far. This one has received more attention than most of these and is in the 52nd percentile.
So far Altmetric has tracked 1,732 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 187,979 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 83 others from the same source and published within six weeks on either side of this one. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.