Title |
Functional emergence of a column-like architecture in layer 5 of mouse somatosensory cortex in vivo
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Published in |
The Journal of Physiological Sciences, May 2018
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DOI | 10.1007/s12576-018-0618-4 |
Pubmed ID | |
Authors |
Kyo Koizumi, Masatoshi Inoue, Srikanta Chowdhury, Haruhiko Bito, Akihiro Yamanaka, Toru Ishizuka, Hiromu Yawo |
Abstract |
To investigate how the functional architecture is organized in layer 5 (L5) of the somatosensory cortex of a mouse in vivo, the input-output relationship was investigated using an all-optical approach. The neural activity in L5 was optically recorded using a Ca2+ sensor, R-CaMP2, through a microprism inserted in the cortex under two-photon microscopy, while the L5 was regionally excited using optogenetics. The excitability was spread around the blue-light irradiated region, but the horizontal propagation was limited to within a certain distance (λ < 130 μm from the center of the illumination spot). When two regions were photostimulated with a short interval, the excitability of each cluster was reduced. Therefore, a column-like architecture had functionally emerged with reciprocal inhibition through a minimal number of synaptic relays. This could generate a synchronous output from a region of L5 with simultaneous enhancement of the signal-to-noise ratio by silencing of the neighboring regions. |
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Other | 1 | 4% |
Unknown | 5 | 22% |