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Application of different techniques to detect Toxoplasma gondii in slaughtered sheep for human consumption

Overview of attention for article published in Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária, December 2015
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Title
Application of different techniques to detect Toxoplasma gondii in slaughtered sheep for human consumption
Published in
Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária, December 2015
DOI 10.1590/s1984-29612015076
Pubmed ID
Authors

Annelise Castanha Barreto Tenório Nunes, Edna Maria Vieira da Silva, José Aelson de Oliveira, Elise Myuki Yamasaki, Pomy de Cássia Peixoto Kim, Jonatas Campos de Almeida, Kleber Barros Nunes, Rinaldo Aparecido Mota

Abstract

The aim of this study was to investigate occurrence of Toxoplasma gondii in sheep slaughtered in the state of Alagoas, Brazil, by means of different diagnosis techniques. Serum samples and tissues from 100 slaughtered sheep were used. To detect antibodies, the indirect immunofluorescence antibody test (IFAT) was used, and tissues from seropositive animals (cut-off ≥1:64) were submitted to Polymerase Chain Reaction (PCR) and immunohistochemistry (IHC). To assess the concordance between the direct techniques, the kappa test was used. In the IFAT, it was observed that 14% (14/100) of the ovine samples were serum-positive. In the PCR, 21.43% (3/14) of the animals were positive and in IHC, it was observed that 7.14% (1/14) were positively stained for T. gondii in cerebral tissue. Histopathologically, the predominant finding was the presence of mononuclear cell infiltrate in the heart and a perivascular cuff in the cerebrum and cerebellum. The concordance between the direct diagnosis techniques was moderate (k=0.44). Thus, it is important to use different direct techniques in diagnosing toxoplasmosis in naturally infected sheep.

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Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Postgraduate 1 8%
Student > Master 1 8%
Unknown 11 85%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 1 8%
Agricultural and Biological Sciences 1 8%
Unknown 11 85%