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PCR melting profile as a tool for outbreak studies of Salmonella enterica in chickens

Overview of attention for article published in BMC Veterinary Research, June 2015
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Title
PCR melting profile as a tool for outbreak studies of Salmonella enterica in chickens
Published in
BMC Veterinary Research, June 2015
DOI 10.1186/s12917-015-0451-4
Pubmed ID
Authors

Anna Zaczek, Arkadiusz Wojtasik, Radosław Izdebski, Elzbieta Gorecka, Ewelina A. Wojcik, Tomasz Nowak, Piotr Kwiecinski, Jaroslaw Dziadek

Abstract

Salmonellosis is of great economic concern in all phases of the poultry industry, from production to marketing, leading to severe economic losses. Monitoring the source of the bacterial contamination has fundamental importance in the spreading of salmonellosis. We applied a ligation-mediated PCR method, PCR MP (PCR melting profile), to type S. enterica ssp. enterica ser. Enteritidis (56 strains) and 43 control strains classified to other serovars isolated from poultry. We demonstrated the PCR MP potential for salmonellosis spreading monitoring. Our rapid test presents higher discriminatory power (0.939 vs. 0.608) compared to current molecular subtyping tool such as pulsed-field gel electrophoresis (PFGE), which ineffectiveness underlies the high degree of clonality of S. Enteritidis. PCR MP was found to be a highly discriminating, sensitive and specific method that could be a valuable molecular tool, particularly for analyzing epidemiological links of limited number of S. enterica ser. Enteritidis strains.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 21%
Student > Ph. D. Student 2 14%
Unspecified 1 7%
Student > Bachelor 1 7%
Professor 1 7%
Other 2 14%
Unknown 4 29%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 36%
Veterinary Science and Veterinary Medicine 2 14%
Biochemistry, Genetics and Molecular Biology 1 7%
Unspecified 1 7%
Unknown 5 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 June 2015.
All research outputs
#18,616,159
of 23,881,329 outputs
Outputs from BMC Veterinary Research
#1,732
of 3,087 outputs
Outputs of similar age
#180,319
of 266,306 outputs
Outputs of similar age from BMC Veterinary Research
#35
of 65 outputs
Altmetric has tracked 23,881,329 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,087 research outputs from this source. They receive a mean Attention Score of 4.2. This one is in the 36th percentile – i.e., 36% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 266,306 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 65 others from the same source and published within six weeks on either side of this one. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.