Title |
Expression patterns of intestinal calcium transport factors and ex-vivo absorption of calcium in horses
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Published in |
BMC Veterinary Research, October 2011
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DOI | 10.1186/1746-6148-7-65 |
Pubmed ID | |
Authors |
Nele Sprekeler, Tobias Müller, Mariusz P Kowalewski, Annette Liesegang, Alois Boos |
Abstract |
In many species, the small intestine is the major site of calcium (Ca(2+)) absorption. The horse differs considerably from most other species with regard to the physiology of its Ca(2+) metabolism and digestion. Thus, this study was performed to get more information about the transcellular Ca(2+) absorption in the horse.Two mechanisms of intestinal Ca(2+) absorption are described: the passive paracellular pathway and the active, vitamin D-dependent transcellular pathway. The latter involves the following elements: vitamin D receptors (VDR), transient receptor potential vanilloid channel members 5 and 6 (TRPV5/6), calbindin-D9k (CB), the Na/Ca exchanger (NCX1) and the plasma membrane Ca-ATPase (PMCA). The aim of the present study was to investigate the protein and mRNA expression patterns of VDR, CB and TRPV6 and the ex-vivo Ca(2+) absorption in horses, assessed by qualitative and quantitative RT-PCR, western blot, immunohistochemistry and the Ussing chamber technique. |
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