↓ Skip to main content

Differences in affinity of monoclonal and naturally acquired polyclonal antibodies against Plasmodium falciparum merozoite antigens

Overview of attention for article published in BMC Microbiology, July 2015
Altmetric Badge

About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (52nd percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
3 tweeters

Citations

dimensions_citation
6 Dimensions

Readers on

mendeley
46 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Differences in affinity of monoclonal and naturally acquired polyclonal antibodies against Plasmodium falciparum merozoite antigens
Published in
BMC Microbiology, July 2015
DOI 10.1186/s12866-015-0461-1
Pubmed ID
Authors

Sreenivasulu B. Reddy, Robin F. Anders, Nadia Cross, Ivo Mueller, Nicolas Senn, Danielle I. Stanisic, Peter M. Siba, Mats Wahlgren, Fred Kironde, James G. Beeson, Kristina E.M. Persson

Abstract

Malaria is a major global cause of deaths and a vaccine is urgently needed. We have employed the P. falciparum merozoite antigens MSP2-3D7/FC27 and AMA1, used them in ELISA, and coupled them in different ways using surface plasmon resonance (SPR) and estimated affinity (measured as kd) of monoclonal as well as naturally-acquired polyclonal antibodies in human plasma. There were major differences in kd depending on how the antigens were immobilized and where the His-tag was placed. For AMA1 we could see correlations with invasion inhibition. Using different immobilizations of proteins in SPR, we could see only moderate correlations with levels of antibodies in ELISA, indicating that in ELISA the proteins were not uniformly bound and that antibodies with many specificities exist in natural immunisation. The correlations between ELISA and SPR were enhanced when only parasite positive samples were included, which may indicate that high affinity antibodies are difficult to maintain over long periods of time. We found higher kd values for MSP2 (indicating lower affinity) compared to AMA1, which might be partly explained by MSP2 being an intrinsically disordered protein, while AMA1 is globular. For future vaccine studies and for understanding immunity, it is important to consider how to present proteins to the immune system to achieve highest antibody affinities.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Kenya 1 2%
Unknown 45 98%

Demographic breakdown

Readers by professional status Count As %
Student > Master 14 30%
Student > Bachelor 8 17%
Researcher 6 13%
Student > Ph. D. Student 6 13%
Student > Doctoral Student 3 7%
Other 6 13%
Unknown 3 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 11 24%
Biochemistry, Genetics and Molecular Biology 10 22%
Immunology and Microbiology 7 15%
Medicine and Dentistry 5 11%
Chemistry 3 7%
Other 6 13%
Unknown 4 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 March 2016.
All research outputs
#3,380,856
of 7,430,207 outputs
Outputs from BMC Microbiology
#523
of 1,259 outputs
Outputs of similar age
#97,712
of 223,417 outputs
Outputs of similar age from BMC Microbiology
#21
of 46 outputs
Altmetric has tracked 7,430,207 research outputs across all sources so far. This one has received more attention than most of these and is in the 52nd percentile.
So far Altmetric has tracked 1,259 research outputs from this source. They receive a mean Attention Score of 2.9. This one has gotten more attention than average, scoring higher than 54% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 223,417 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one is in the 43rd percentile – i.e., 43% of its contemporaries scored the same or lower than it.