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Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart

Overview of attention for article published in Brazilian oral research, May 2018
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Title
Antifungal, antibiofilm, and antiproliferative activities of Guapira graciliflora Mart
Published in
Brazilian oral research, May 2018
DOI 10.1590/1807-3107bor-2018.vol32.0041
Pubmed ID
Authors

Carolina Medeiros de Almeida, Rennaly de Freitas Lima, Tereza Karla Vieira Lopes da Costa, Ilza Maria de Oliveira Sousa, Elaine Cristina Cabral, Rosanna Tarkany Basting, Adriana Della Torre, Yuri Wanderley Cavalcanti, Pedro Luiz Rosalen, Marta Cristina Teixeira Duarte, Ana Lúcia Tasca Gois Ruiz, Mary Ann Foglio, Gustavo Pina Godoy, Edja Maria Melo de Brito Costa

Abstract

The aim of this study was to evaluate in vitro the antifungal, antibiofilm and antiproliferative activities of the extract from the leaves of Guapira graciliflora Mart. The phytochemical characterization of the extract was performed using electrospray ionization mass spectrometry (ESI-MS). The antimicrobial activity of the extract and its fractions was evaluated using the broth microdilution method against species of Candida. The inhibition of C. albicans biofilm was evaluated based on the number of colony-forming units (CFU) and metabolic activity (MTT). The antiproliferative activity of the extract and its fraction was evaluated in the presence of human tumor and non-tumor cells, and the cytotoxicity of the extract was determined on the RAW 264.7 macrophage line - both using the sulforhodamine B method. The phytochemical characterization indicated the presence of the flavonoids rutin and kaempferol. The extract and the methanol fraction exhibited moderate antifungal activity against C. albicans, C. krusei, and C. glabrata, and strong activity against C. dubliniensis. In the biofilms at 24 and 48 hours, the concentration of 12500 µg/mL of the extract was the most effective at reducing the number of CFU s/mL (44.4% and 42.9%, respectively) and the metabolic activity of C. albicans cells (34.6% and 52%, respectively). The extract and its fractions had no antiproliferative effect on the tumor lines tested, with mean activity (log GI50) equal to or greater than 1.71 µg/mL. Macrophage cell viability remained higher than 80% for concentrations of the extract of up to 62.5 µg/mL. G. graciliflora has flavonoids in its chemical composition and demonstrates potential antifungal and antibiofilm activity, with no evidence of a significant change in the viability of human tumor and non-tumor cell lines.

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Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Postgraduate 2 12%
Professor 2 12%
Professor > Associate Professor 2 12%
Student > Master 1 6%
Researcher 1 6%
Other 1 6%
Unknown 8 47%
Readers by discipline Count As %
Immunology and Microbiology 4 24%
Pharmacology, Toxicology and Pharmaceutical Science 2 12%
Agricultural and Biological Sciences 1 6%
Unknown 10 59%