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Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil

Overview of attention for article published in Revista Brasileira de Parasitologia Veterinária, May 2018
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  • Good Attention Score compared to outputs of the same age and source (68th percentile)

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32 Mendeley
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Title
Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil
Published in
Revista Brasileira de Parasitologia Veterinária, May 2018
DOI 10.1590/s1984-296120180028
Pubmed ID
Authors

Tamiris Cristine Ribeiro Ferreira, Julyana Cerqueira Buery, Narcisa Imaculada Brant Moreira, Claudiney Biral Santos, Júlia Gatti Ladeia Costa, Lorena Velozo Pinto, Ramon Castro de Araújo Baraviera, Ricardo Wagner Almeida Vitor, Blima Fux

Abstract

Toxoplasma gondii presents a high prevalence worldwide, infecting several animals. Felines are considered the definitive hosts and among the intermediate hosts we highlight mammals and birds. The man can become infected by ingesting tissue cysts present in birds and mammals. Biological and molecular aspects of T. gondii allows a better understanding of the epidemiology of toxoplasmosis. This work is a serologic screening of 58 chickens grown (Gallus gallus domesticus) for human consumption in Espírito Santo State, by means of indirect haemagglutination assay (IHA). Thirteen chickens tested positive for anti-T. gondii antibodies. The heart and brain of five positive chickens were harvested, treated with pepsin and inoculated separately, in two Swiss mice, intraperitoneally. Tachyzoites were observed in the peritoneum of all the animals, between seven and 10 days after the inoculum. Ten isolates were obtained and biologically characterised in BALB/c mice inoculated with 101 to 104 tachyzoites. All isolates were classified as virulent or intermediately virulent. Isolates were genotyped by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, revealing three different genotypes. None of the isolates exhibited the clonal type I, II or III genotype. No genotypic differences were observed between the isolates from the brain or heart from the same bird.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 6 19%
Other 3 9%
Student > Doctoral Student 3 9%
Student > Bachelor 3 9%
Researcher 3 9%
Other 2 6%
Unknown 12 38%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 7 22%
Agricultural and Biological Sciences 4 13%
Biochemistry, Genetics and Molecular Biology 3 9%
Nursing and Health Professions 1 3%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Other 2 6%
Unknown 14 44%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 June 2019.
All research outputs
#17,292,294
of 25,382,440 outputs
Outputs from Revista Brasileira de Parasitologia Veterinária
#206
of 660 outputs
Outputs of similar age
#222,147
of 344,113 outputs
Outputs of similar age from Revista Brasileira de Parasitologia Veterinária
#5
of 19 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 660 research outputs from this source. They receive a mean Attention Score of 1.2. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 344,113 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.