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Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil

Overview of attention for article published in Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, May 2018
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Title
Toxoplasma gondii: isolation, biological and molecular characterisation of samples from free-range Gallus gallus domesticus from countryside Southeast Brazil
Published in
Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, May 2018
DOI 10.1590/s1984-296120180028
Pubmed ID
Authors

Tamiris Cristine Ribeiro Ferreira, Julyana Cerqueira Buery, Narcisa Imaculada Brant Moreira, Claudiney Biral Santos, Júlia Gatti Ladeia Costa, Lorena Velozo Pinto, Ramon Castro de Araújo Baraviera, Ricardo Wagner Almeida Vitor, Blima Fux

Abstract

Toxoplasma gondii presents a high prevalence worldwide, infecting several animals. Felines are considered the definitive hosts and among the intermediate hosts we highlight mammals and birds. The man can become infected by ingesting tissue cysts present in birds and mammals. Biological and molecular aspects of T. gondii allows a better understanding of the epidemiology of toxoplasmosis. This work is a serologic screening of 58 chickens grown (Gallus gallus domesticus) for human consumption in Espírito Santo State, by means of indirect haemagglutination assay (IHA). Thirteen chickens tested positive for anti-T. gondii antibodies. The heart and brain of five positive chickens were harvested, treated with pepsin and inoculated separately, in two Swiss mice, intraperitoneally. Tachyzoites were observed in the peritoneum of all the animals, between seven and 10 days after the inoculum. Ten isolates were obtained and biologically characterised in BALB/c mice inoculated with 101 to 104 tachyzoites. All isolates were classified as virulent or intermediately virulent. Isolates were genotyped by means of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, revealing three different genotypes. None of the isolates exhibited the clonal type I, II or III genotype. No genotypic differences were observed between the isolates from the brain or heart from the same bird.

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Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 16%
Student > Master 4 16%
Other 3 12%
Researcher 3 12%
Student > Ph. D. Student 1 4%
Other 2 8%
Unknown 8 32%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 5 20%
Biochemistry, Genetics and Molecular Biology 3 12%
Agricultural and Biological Sciences 3 12%
Medicine and Dentistry 2 8%
Nursing and Health Professions 1 4%
Other 1 4%
Unknown 10 40%