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Impact of nanosilver on various DNA lesions and HPRT gene mutations – effects of charge and surface coating

Overview of attention for article published in Particle and Fibre Toxicology, July 2015
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2 tweeters

Citations

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35 Mendeley
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Title
Impact of nanosilver on various DNA lesions and HPRT gene mutations – effects of charge and surface coating
Published in
Particle and Fibre Toxicology, July 2015
DOI 10.1186/s12989-015-0100-x
Pubmed ID
Authors

Anna Huk, Emilia Izak-Nau, Naouale el Yamani, Hilde Uggerud, Marit Vadset, Beata Zasonska, Albert Duschl, Maria Dusinska

Abstract

The main goal of this research was to study the interactions of a fully characterized set of silver nanomaterials (Ag ENMs) with cells in vitro, according to the standards of Good Laboratory Practices (GLP), to assure the quality of nanotoxicology research. We were interested in whether Ag ENMs synthesized by the same method, with the same size distribution, shape and specific surface area, but with different charges and surface compositions could give different biological responses. A range of methods and toxicity endpoints were applied to study the impacts of interaction of the Ag ENMs with TK6 cells. As tests of viability, relative growth activity and trypan blue exclusion were applied. Genotoxicity was evaluated by the alkaline comet assay for detection of strand breaks and oxidized purines. The mutagenic potential of Ag ENMs was investigated with the in vitro HPRT gene mutation test on V79-4 cells according to the OECD protocol. Ag ENM agglomeration, dissolution as well as uptake and distribution within the cells were investigated as crucial aspects of Ag ENM toxicity. Ag ENM stabilizers were included in addition to positive and negative controls. Different cytotoxic effects were observed including membrane damage, cell cycle arrest and cell death. Ag ENMs also induced various kinds of DNA damage including strand breaks and DNA oxidation, and caused gene mutation. We found that positive Ag ENMs had greater impact on cyto- and genotoxicity than did Ag ENMs with neutral or negative charge, assumed to be related to their greater uptake into cells and to their presence in the nucleus and mitochondria, implying that Ag ENMs might induce toxicity by both direct and indirect mechanisms. We showed that Ag ENMs could be cytotoxic, genotoxic and mutagenic. Our experiments with the HPRT gene mutation assay demonstrated that surface chemical composition plays a significant role in Ag ENM toxicity.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 3%
Russia 1 3%
Austria 1 3%
Unknown 32 91%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 20%
Researcher 7 20%
Student > Ph. D. Student 5 14%
Student > Bachelor 5 14%
Other 3 9%
Other 6 17%
Unknown 2 6%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 14%
Biochemistry, Genetics and Molecular Biology 5 14%
Pharmacology, Toxicology and Pharmaceutical Science 3 9%
Chemistry 3 9%
Physics and Astronomy 3 9%
Other 10 29%
Unknown 6 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 September 2015.
All research outputs
#4,534,747
of 8,859,380 outputs
Outputs from Particle and Fibre Toxicology
#159
of 302 outputs
Outputs of similar age
#113,500
of 231,791 outputs
Outputs of similar age from Particle and Fibre Toxicology
#11
of 13 outputs
Altmetric has tracked 8,859,380 research outputs across all sources so far. This one is in the 46th percentile – i.e., 46% of other outputs scored the same or lower than it.
So far Altmetric has tracked 302 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 231,791 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.