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An Enriched European Eel Transcriptome Sheds Light upon Host-Pathogen Interactions with Vibrio vulnificus

Overview of attention for article published in PLoS ONE, July 2015
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Title
An Enriched European Eel Transcriptome Sheds Light upon Host-Pathogen Interactions with Vibrio vulnificus
Published in
PLoS ONE, July 2015
DOI 10.1371/journal.pone.0133328
Pubmed ID
Authors

Agnès Callol, Felipe E. Reyes-López, Francisco J. Roig, Giles Goetz, Frederick W. Goetz, Carmen Amaro, Simon A. MacKenzie

Abstract

Infectious diseases are one of the principal bottlenecks for the European eel recovery. The aim of this study was to develop a new molecular tool to be used in host-pathogen interaction experiments in the eel. To this end, we first stimulated adult eels with different pathogen-associated molecular patterns (PAMPs), extracted RNA from the immune-related tissues and sequenced the transcriptome. We obtained more than 2x106 reads that were assembled and annotated into 45,067 new descriptions with a notable representation of novel transcripts related with pathogen recognition, signal transduction and the immune response. Then, we designed a DNA-microarray that was used to analyze the early immune response against Vibrio vulnificus, a septicemic pathogen that uses the gills as the portal of entry into the blood, as well as the role of the main toxin of this species (RtxA13) on this early interaction. The gill transcriptomic profiles obtained after bath infecting eels with the wild type strain or with a mutant deficient in rtxA13 were analyzed and compared. Results demonstrate that eels react rapidly and locally against the pathogen and that this immune-response is rtxA13-dependent as transcripts related with cell destruction were highly up-regulated only in the gills from eels infected with the wild-type strain. Furthermore, significant differences in the immune response against the wild type and the mutant strain also suggest that host survival after V. vulnificus infection could depend on an efficient local phagocytic activity. Finally, we also found evidence of the presence of an interbranchial lymphoid tissue in European eel gills although further experiments will be necessary to identify such tissue.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 5%
Unknown 21 95%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 45%
Student > Master 3 14%
Student > Ph. D. Student 3 14%
Student > Bachelor 2 9%
Other 1 5%
Other 3 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 32%
Biochemistry, Genetics and Molecular Biology 4 18%
Unspecified 3 14%
Immunology and Microbiology 3 14%
Economics, Econometrics and Finance 2 9%
Other 3 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 July 2015.
All research outputs
#6,073,241
of 7,063,725 outputs
Outputs from PLoS ONE
#87,157
of 103,419 outputs
Outputs of similar age
#185,288
of 224,238 outputs
Outputs of similar age from PLoS ONE
#5,241
of 6,214 outputs
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