Title |
Effects of karanjin on cell cycle arrest and apoptosis in human A549, HepG2 and HL-60 cancer cells
|
---|---|
Published in |
Biological Research, July 2015
|
DOI | 10.1186/s40659-015-0031-x |
Pubmed ID | |
Authors |
Jian-Ru Guo, Qian-Qian Chen, Christopher Wai-Kei Lam, Wei Zhang |
Abstract |
We have investigated the potential anticancer effects of karanjin, a principal furanoflavonol constituent of the Chinese medicine Fordia cauliflora, using cytotoxic assay, cell cycle arrest, and induction of apoptosis in three human cancer cell lines (A549, HepG2 and HL-60 cells). MTT cytotoxic assay showed that karanjin could inhibit the proliferation and viability of all three cancer cells. The induction of cell cycle arrest was observed via a PI (propidium iodide)/RNase Staining Buffer detection kit and analyzed by flow cytometry: karanjin could dose-dependently induce cell cycle arrest at G2/M phase in the three cell lines. Cell apoptosis was assessed by Annexin V-FITC/PI staining: all three cancer cells treated with karanjin exhibited significantly increased apoptotic rates, especially in the percentage of late apoptosis cells. Karanjin can induce cancer cell death through cell cycle arrest and enhance apoptosis. This compound may be effective clinically for cancer pharmacotherapy. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 28 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 18% |
Student > Postgraduate | 3 | 11% |
Student > Master | 3 | 11% |
Other | 2 | 7% |
Professor > Associate Professor | 2 | 7% |
Other | 3 | 11% |
Unknown | 10 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 21% |
Medicine and Dentistry | 3 | 11% |
Chemistry | 2 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Energy | 1 | 4% |
Other | 3 | 11% |
Unknown | 12 | 43% |