Title |
deFUME: Dynamic exploration of functional metagenomic sequencing data
|
---|---|
Published in |
BMC Research Notes, July 2015
|
DOI | 10.1186/s13104-015-1281-y |
Pubmed ID | |
Authors |
Eric van der Helm, Henrik Marcus Geertz-Hansen, Hans Jasper Genee, Sailesh Malla, Morten Otto Alexander Sommer |
Abstract |
Functional metagenomic selections represent a powerful technique that is widely applied for identification of novel genes from complex metagenomic sources. However, whereas hundreds to thousands of clones can be easily generated and sequenced over a few days of experiments, analyzing the data is time consuming and constitutes a major bottleneck for experimental researchers in the field. Here we present the deFUME web server, an easy-to-use web-based interface for processing, annotation and visualization of functional metagenomics sequencing data, tailored to meet the requirements of non-bioinformaticians. The web-server integrates multiple analysis steps into one single workflow: read assembly, open reading frame prediction, and annotation with BLAST, InterPro and GO classifiers. Analysis results are visualized in an online dynamic web-interface. The deFUME webserver provides a fast track from raw sequence to a comprehensive visual data overview that facilitates effortless inspection of gene function, clustering and distribution. The webserver is available at cbs.dtu.dk/services/deFUME/and the source code is distributed at github.com/EvdH0/deFUME. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 6 | 32% |
United Kingdom | 2 | 11% |
Canada | 1 | 5% |
Finland | 1 | 5% |
Germany | 1 | 5% |
Denmark | 1 | 5% |
Unknown | 7 | 37% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 10 | 53% |
Scientists | 9 | 47% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Brazil | 2 | 4% |
Portugal | 1 | 2% |
Denmark | 1 | 2% |
Estonia | 1 | 2% |
Spain | 1 | 2% |
Unknown | 44 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 18 | 36% |
Researcher | 6 | 12% |
Student > Master | 5 | 10% |
Student > Bachelor | 5 | 10% |
Student > Doctoral Student | 3 | 6% |
Other | 9 | 18% |
Unknown | 4 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 16 | 32% |
Biochemistry, Genetics and Molecular Biology | 11 | 22% |
Computer Science | 5 | 10% |
Engineering | 3 | 6% |
Environmental Science | 2 | 4% |
Other | 5 | 10% |
Unknown | 8 | 16% |