Title |
Extracellular vesicles direct migration by synthesizing and releasing chemotactic signals
|
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Published in |
Journal of Cell Biology, June 2018
|
DOI | 10.1083/jcb.201710170 |
Pubmed ID | |
Authors |
Paul W. Kriebel, Ritankar Majumdar, Lisa M. Jenkins, Hiroshi Senoo, Weiye Wang, Sonia Ammu, Song Chen, Kedar Narayan, Miho Iijima, Carole A. Parent |
Abstract |
Chemotactic signals are relayed to neighboring cells through the secretion of additional chemoattractants. We previously showed in Dictyostelium discoideum that the adenylyl cyclase A, which synthesizes the chemoattractant cyclic adenosine monophosphate (cAMP), is present in the intraluminal vesicles of multivesicular bodies (MVBs) that coalesce at the back of cells. Using ultrastructural reconstructions, we now show that ACA-containing MVBs release their contents to attract neighboring cells. We show that the released vesicles are capable of directing migration and streaming and are central to chemotactic signal relay. We demonstrate that the released vesicles not only contain cAMP but also can actively synthesize and release cAMP to promote chemotaxis. Through proteomic, pharmacological, and genetic approaches, we determined that the vesicular cAMP is released via the ABCC8 transporter. Together, our findings show that extracellular vesicles released by Ddiscoideum cells are functional entities that mediate signal relay during chemotaxis and streaming. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 31 | 40% |
United Kingdom | 8 | 10% |
India | 2 | 3% |
Mexico | 2 | 3% |
Germany | 2 | 3% |
Austria | 2 | 3% |
Korea, Republic of | 1 | 1% |
Iran, Islamic Republic of | 1 | 1% |
Canada | 1 | 1% |
Other | 7 | 9% |
Unknown | 21 | 27% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 44 | 56% |
Scientists | 29 | 37% |
Science communicators (journalists, bloggers, editors) | 4 | 5% |
Practitioners (doctors, other healthcare professionals) | 1 | 1% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 109 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 33 | 30% |
Researcher | 15 | 14% |
Student > Master | 11 | 10% |
Student > Doctoral Student | 7 | 6% |
Student > Bachelor | 7 | 6% |
Other | 13 | 12% |
Unknown | 23 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 46 | 42% |
Agricultural and Biological Sciences | 14 | 13% |
Immunology and Microbiology | 6 | 6% |
Medicine and Dentistry | 5 | 5% |
Engineering | 3 | 3% |
Other | 10 | 9% |
Unknown | 25 | 23% |