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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Infrared spectroscopy reveals multi-step multi-timescale photoactivation in the photoconvertible protein archetype dronpa
|
|---|---|
| Published in |
Nature Chemistry, June 2018
|
| DOI | 10.1038/s41557-018-0073-0 |
| Pubmed ID | |
| Authors |
Sergey P. Laptenok, Agnieszka A. Gil, Christopher R. Hall, Andras Lukacs, James N. Iuliano, Garth A. Jones, Gregory M. Greetham, Paul Donaldson, Atsushi Miyawaki, Peter J. Tonge, Stephen R. Meech |
| Abstract |
Photochromic fluorescent proteins play key roles in super-resolution microscopy and optogenetics. The light-driven structural changes that modulate the fluorescence involve both trans-to-cis isomerization and proton transfer. The mechanism, timescale and relative contribution of chromophore and protein dynamics are currently not well understood. Here, the mechanism of off-to-on-state switching in dronpa is studied using femtosecond-to-millisecond time-resolved infrared spectroscopy and isotope labelling. Chromophore and protein dynamics are shown to occur on multiple timescales, from picoseconds to hundreds of microseconds. Following excitation of the trans chromophore, a ground-state primary product is formed within picoseconds. Surprisingly, the characteristic vibrational spectrum of the neutral cis isomer appears only after several tens of nanoseconds. Further fluctuations in protein structure around the neutral cis chromophore are required to form a new intermediate, which promotes the final proton-transfer reaction. These data illustrate the interplay between chromophore dynamics and the protein environment underlying fluorescent protein photochromism. |
X Demographics
The data shown below were collected from the profiles of 22 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 3 | 14% |
| United Kingdom | 3 | 14% |
| United States | 2 | 9% |
| Mexico | 2 | 9% |
| Italy | 1 | 5% |
| Indonesia | 1 | 5% |
| Unknown | 10 | 45% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 14 | 64% |
| Scientists | 8 | 36% |
Mendeley readers
The data shown below were compiled from readership statistics for 74 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 74 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 19 | 26% |
| Researcher | 18 | 24% |
| Other | 6 | 8% |
| Student > Master | 6 | 8% |
| Professor | 6 | 8% |
| Other | 10 | 14% |
| Unknown | 9 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 30 | 41% |
| Biochemistry, Genetics and Molecular Biology | 10 | 14% |
| Agricultural and Biological Sciences | 8 | 11% |
| Physics and Astronomy | 7 | 9% |
| Unspecified | 2 | 3% |
| Other | 4 | 5% |
| Unknown | 13 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 32. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 April 2019.
All research outputs
#1,218,799
of 25,346,731 outputs
Outputs from Nature Chemistry
#1,003
of 3,320 outputs
Outputs of similar age
#25,844
of 335,452 outputs
Outputs of similar age from Nature Chemistry
#25
of 57 outputs
Altmetric has tracked 25,346,731 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,320 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 36.0. This one has gotten more attention than average, scoring higher than 69% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 335,452 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 92% of its contemporaries.
We're also able to compare this research output to 57 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 57% of its contemporaries.