Title |
One-Step Bioprocess of Inulin to Product Inulo-Oligosaccharides Using Bacillus subtilis Secreting an Extracellular Endo-Inulinase
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Published in |
Applied Biochemistry and Biotechnology, June 2018
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DOI | 10.1007/s12010-018-2806-3 |
Pubmed ID | |
Authors |
Ruifan Jiang, Yibin Qiu, Weiwei Huang, Li Zhang, Feng Xue, Hao Ni, Difen Mei, Jian Gao, Hong Xu |
Abstract |
Inulo-oligosaccharides (IOSs), a novel food additive and health product, represent a promising alternative to antibiotics. As prebiotics, IOSs can be obtained from inulin by endo-inulinase-mediated hydrolysis. Nonetheless, enzymatic catalysis is not feasible industrially because of the required catalytic conditions and cost. In this study, a 2331-bp optimized gene inuQ (from Pseudomonas mucidolens) encoding endo-inulinase was cloned into shuttle vector PHY300PLK and transfected into Bacillus subtilis WB800-R, with the simultaneous deletion of gene sacC encoding levanase. The maximal IOS yield after hydrolysis of the crude extract of inulin was 67.84 ± 0.72 g/L for a recombinant strain with the signal peptide nprB from alkaline protease and promoter P43. The conversion rate reached 75.38%. For the major IOSs, the degree of polymerization was between 3 and 5. This study offers a simple and efficient one-step bioprocess for IOS production from inulin through secretion of an extracellular heterologous endo-inulinase by B. subtilis. |
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Unknown | 2 | 100% |
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Members of the public | 2 | 100% |
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Researcher | 3 | 17% |
Student > Doctoral Student | 2 | 11% |
Other | 2 | 11% |
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Social Sciences | 1 | 6% |
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Unknown | 5 | 28% |