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Mendeley readers
Attention Score in Context
| Title |
Resurrection of a Bull by Cloning from Organs Frozen without Cryoprotectant in a −80°C Freezer for a Decade
|
|---|---|
| Published in |
PLOS ONE, January 2009
|
| DOI | 10.1371/journal.pone.0004142 |
| Pubmed ID | |
| Authors |
Yoichiro Hoshino, Noboru Hayashi, Shunji Taniguchi, Naohiko Kobayashi, Kenji Sakai, Tsuyoshi Otani, Akira Iritani, Kazuhiro Saeki |
| Abstract |
Frozen animal tissues without cryoprotectant have been thought to be inappropriate for use as a nuclear donor for somatic cell nuclear transfer (SCNT). We report the cloning of a bull using cells retrieved from testicles that had been taken from a dead animal and frozen without cryoprotectant in a -80 degrees C freezer for 10 years. We obtained live cells from defrosted pieces of the spermatic cords of frozen testicles. The cells proliferated actively in culture and were apparently normal. We transferred 16 SCNT embryos from these cells into 16 synchronized recipient animals. We obtained five pregnancies and four cloned calves developed to term. Our results indicate that complete genome sets are maintained in mammalian organs even after long-term frozen-storage without cryoprotectant, and that live clones can be produced from the recovered cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 3% |
| United Kingdom | 1 | 3% |
| Unknown | 32 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 29% |
| Student > Bachelor | 8 | 24% |
| Other | 4 | 12% |
| Student > Ph. D. Student | 4 | 12% |
| Student > Master | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 4 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 47% |
| Medicine and Dentistry | 6 | 18% |
| Biochemistry, Genetics and Molecular Biology | 4 | 12% |
| Philosophy | 1 | 3% |
| Computer Science | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 3 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 47. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 November 2020.
All research outputs
#743,003
of 22,656,971 outputs
Outputs from PLOS ONE
#10,407
of 193,432 outputs
Outputs of similar age
#2,517
of 169,156 outputs
Outputs of similar age from PLOS ONE
#33
of 457 outputs
Altmetric has tracked 22,656,971 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 193,432 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.0. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 169,156 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 98% of its contemporaries.
We're also able to compare this research output to 457 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.