Title |
Tumor-targeting, pH-sensitive nanoparticles for docetaxel delivery to drug-resistant cancer cells
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Published in |
International Journal of Nanomedicine, August 2015
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DOI | 10.2147/ijn.s89584 |
Pubmed ID | |
Authors |
Tuan Hiep Tran, Thiruganesh Ramasamy, Ju Yeon Choi, Hanh Thuy Nguyen, Thanh Tung Pham, Jee-Heon Jeong, Sae Kwang Ku, Han-Gon Choi, Chul Soon Yong, Jong Oh Kim |
Abstract |
The attachment of polyethylene glycol (PEG) increases the circulation time of drug-containing nanoparticles; however, this also negatively affects cellular uptake. To overcome this problem, unique lipid polymer hybrid (LPH) nanoparticles were developed with a pH-responsive PEG layer that detached prior to cell uptake. Docetaxel (DTX) was incorporated into the lipid core of the nanoparticles, which was then shielded with the pH-responsive block co-polymer polyethylene glycol-b-polyaspartic acid (PEG-b-PAsp) using a modified emulsion method. The optimized LPH nanoparticles were ~200 nm and had a narrow size distribution. Drug release from DTX-loaded LPH (DTX-LPH) nanoparticles was pH-sensitive, which is beneficial for tumor targeting. More importantly, DTX-LPH nanoparticles were able to effectively induce apoptosis in cancer cells. The negative surface charge and PEG shell of vehicle remarkably enhanced the blood circulation and physiological activity of DTX-LPH nanoparticles compared with that of free DTX. The nanoparticles were also found to reduce the size of tumors in tumor-bearing xenograft mice. The in vivo anticancer effect of DTX-LPH nanoparticles was further confirmed by the elevated levels of caspase-3 and poly ADP ribose polymerase found in the tumors after treatment. Thus, the results suggest that this novel LPH system could be an effective new treatment for cancer. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Canada | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Practitioners (doctors, other healthcare professionals) | 2 | 67% |
Members of the public | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 39 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 9 | 23% |
Student > Master | 8 | 21% |
Researcher | 7 | 18% |
Student > Bachelor | 3 | 8% |
Student > Postgraduate | 2 | 5% |
Other | 6 | 15% |
Unknown | 4 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 10 | 26% |
Medicine and Dentistry | 6 | 15% |
Biochemistry, Genetics and Molecular Biology | 4 | 10% |
Agricultural and Biological Sciences | 4 | 10% |
Chemistry | 3 | 8% |
Other | 8 | 21% |
Unknown | 4 | 10% |