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Chapter title |
Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative Immunofluorescence
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Chapter number | 9 |
Book title |
Cell Cycle Oscillators
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Published in |
Methods in molecular biology, January 2016
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DOI | 10.1007/978-1-4939-2957-3_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2956-6, 978-1-4939-2957-3
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Authors |
Karen Akopyan, Arne Lindqvist, Erik Müllers |
Abstract |
Immunofluorescence can be a powerful tool to detect protein levels, intracellular localization, and post-translational modifications. However, standard immunofluorescence provides only a still picture and thus lacks temporal information. Here, we describe a method to extract temporal information from immunofluorescence images of fixed cells. In addition, we provide an optional protocol that uses micropatterns, which increases the accuracy of the method. These methods allow assessing how protein levels, intracellular localization, and post-translational modifications change through the cell cycle. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 50% |
Researcher | 2 | 33% |
Student > Ph. D. Student | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 83% |
Agricultural and Biological Sciences | 1 | 17% |