↓ Skip to main content

Selective repression of RET proto-oncogene in medullary thyroid carcinoma by a natural alkaloid berberine

Overview of attention for article published in BMC Cancer, August 2015
Altmetric Badge

About this Attention Score

  • Good Attention Score compared to outputs of the same age (65th percentile)
  • High Attention Score compared to outputs of the same age and source (82nd percentile)

Mentioned by

twitter
3 tweeters

Citations

dimensions_citation
15 Dimensions

Readers on

mendeley
17 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Selective repression of RET proto-oncogene in medullary thyroid carcinoma by a natural alkaloid berberine
Published in
BMC Cancer, August 2015
DOI 10.1186/s12885-015-1610-5
Pubmed ID
Authors

Vishnu Muthuraj Kumarasamy, Yoon-Joo Shin, John White, Daekyu Sun

Abstract

The gain-of-function mutation of the RET proto-oncogene, which encodes a receptor tyrosine kinase, is strongly associated with the development of several medullary thyroid carcinomas (MTCs). Thus, the RET protein has been explored as an excellent target for progressive and advanced MTC. In this study we have demonstrated a therapeutic strategy for MTC by suppressing the transcription of RET proto-oncogene though the stabilization of G-quadruplex structure formed on the promoter region of this gene using a natural product berberine. Medullary thyroid carcinoma (MTC) TT cell line has been used to evaluate the effects of berberine on RET expression and its downstream signaling pathways. The specificity of berberine was demonstrated by using the papillary thyroid carcinoma TPC1 cell line, which lacks the G-quadruplex forming sequence on the RET promoter region due to chromosomal rearrangement. Berberine suppressed the RET expression by more than 90 % in MTC TT cells at a concentration of 2.5 μg/ml with minimal effect on the TPC1 cells. Canadine, which is a structural analogue of berberine, showed little interaction with RET G-quadruplex and also had no effect on RET expression in MTC TT cells. The down-regulation of RET with berberine further inhibited the cell proliferation through cell cycle arrest and activation of apoptosis in TT cells, which was confirmed by a 2-fold increase in the caspase-3 activity and the down-regulation of cell-cycle regulatory proteins. Our data strongly suggest that the G-quadruplex forming region and the stabilization of this structure play a critical role in mediating the repressive effect of berberine on RET transcription.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 29%
Student > Bachelor 3 18%
Student > Doctoral Student 2 12%
Unspecified 1 6%
Other 1 6%
Other 3 18%
Unknown 2 12%
Readers by discipline Count As %
Medicine and Dentistry 6 35%
Biochemistry, Genetics and Molecular Biology 3 18%
Chemistry 3 18%
Agricultural and Biological Sciences 1 6%
Unknown 4 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 April 2016.
All research outputs
#2,919,564
of 7,492,829 outputs
Outputs from BMC Cancer
#738
of 3,269 outputs
Outputs of similar age
#75,331
of 227,939 outputs
Outputs of similar age from BMC Cancer
#22
of 143 outputs
Altmetric has tracked 7,492,829 research outputs across all sources so far. This one has received more attention than most of these and is in the 60th percentile.
So far Altmetric has tracked 3,269 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 227,939 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 143 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.