Chapter title |
Measuring Innate Immune Function in Mouse Mononuclear Phagocytes
|
---|---|
Chapter number | 11 |
Book title |
Lung Innate Immunity and Inflammation
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-8570-8_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-8569-2, 978-1-4939-8570-8
|
Authors |
John Matthew Craig, Neil Raj Aggarwal, Craig, John Matthew, Aggarwal, Neil Raj |
Abstract |
While serving as a conduit for gas exchange, the lung continually encounters potentially harmful airborne and bloodborne substances including particulate matter, allergens, toxins, and infectious agents. Resident alveolar and interstitial macrophages coordinate with neutrophils, dendritic cells, and recruited blood-derived monocytes to provide phagocytic host defense that aids in the removal and destruction of antigenic material following this myriad of exposures. Here we describe flow cytometric methods for specifically assessing phagocytic activity ex vivo in isolated mouse lung macrophages and monocytes utilizing fluorescently labeled Streptococcus pneumoniae. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 1 | 20% |
Student > Bachelor | 1 | 20% |
Researcher | 1 | 20% |
Other | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Environmental Science | 1 | 20% |
Biochemistry, Genetics and Molecular Biology | 1 | 20% |
Medicine and Dentistry | 1 | 20% |
Materials Science | 1 | 20% |
Unknown | 1 | 20% |