Title |
No evidence of the Shiga toxin-producing E. coli O104:H4 outbreak strain or enteroaggregative E. coli (EAEC) found in cattle faeces in northern Germany, the hotspot of the 2011 HUS outbreak area
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Published in |
Gut Pathogens, November 2011
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DOI | 10.1186/1757-4749-3-17 |
Pubmed ID | |
Authors |
Lothar H Wieler, Torsten Semmler, Inga Eichhorn, Esther M Antao, Bianca Kinnemann, Lutz Geue, Helge Karch, Sebastian Guenther, Astrid Bethe |
Abstract |
Ruminants, in particular bovines, are the primary reservoir of Shiga toxin-producing E. coli (STEC), but whole genome analyses of the current German ESBL-producing O104:H4 outbreak strain of sequence type (ST) 678 showed this strain to be highly similar to enteroaggregative E. coli (EAEC). Strains of the EAEC pathotype are basically adapted to the human host. To clarify whether in contrast to this paradigm, the O104:H4 outbreak strain and/or EAEC may also be able to colonize ruminants, we screened a total of 2.000 colonies from faecal samples of 100 cattle from 34 different farms - all located in the HUS outbreak region of Northern Germany - for genes associated with the O104:H4 HUS outbreak strain (stx2, terD, rfbO104, fliCH4), STEC (stx1, stx2, escV), EAEC (pAA, aggR, astA), and ESBL-production (blaCTX-M, blaTEM, blaSHV). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 2% |
Sweden | 1 | 2% |
Unknown | 47 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 12 | 24% |
Researcher | 11 | 22% |
Student > Postgraduate | 5 | 10% |
Student > Bachelor | 4 | 8% |
Professor > Associate Professor | 3 | 6% |
Other | 9 | 18% |
Unknown | 5 | 10% |
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Biochemistry, Genetics and Molecular Biology | 4 | 8% |
Veterinary Science and Veterinary Medicine | 3 | 6% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
Other | 6 | 12% |
Unknown | 3 | 6% |