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Glutathione depletion triggers actin cytoskeleton changes via actin-binding proteins

Overview of attention for article published in Genetics and Molecular Biology, June 2018
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Title
Glutathione depletion triggers actin cytoskeleton changes via actin-binding proteins
Published in
Genetics and Molecular Biology, June 2018
DOI 10.1590/1678-4685-gmb-2017-0158
Pubmed ID
Authors

Nahum Zepeta-Flores, Mahara Valverde, Alejandro Lopez-Saavedra, Emilio Rojas

Abstract

The importance of glutathione (GSH) in alternative cellular roles to the canonically proposed, were analyzed in a model unable to synthesize GSH. Gene expression analysis shows that the regulation of the actin cytoskeleton pathway is strongly impacted by the absence of GSH. To test this hypothesis, we evaluate the effect of GSH depletion via buthionine sulfoximine (5 and 12.5 mM) in human neuroblastoma MSN cells. In the present study, 70% of GSH reduction did not induce reactive oxygen species, lipoperoxidation, or cytotoxicity, which enabled us to evaluate the effect of glutathione in the absence of oxidative stress. The cells with decreasing GSH levels acquired morphology changes that depended on the actin cytoskeleton and not on tubulin. We evaluated the expression of three actin-binding proteins: thymosin β4, profilin and gelsolin, showing a reduced expression, both at gene and protein levels at 24 hours of treatment; however, this suppression disappears after 48 hours of treatment. These changes were sufficient to trigger the co-localization of the three proteins towards cytoplasmic projections. Our data confirm that a decrease in GSH in the absence of oxidative stress can transiently inhibit the actin binding proteins and that this stimulus is sufficient to induce changes in cellular morphology via the actin cytoskeleton.

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Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 19%
Student > Doctoral Student 2 13%
Student > Bachelor 2 13%
Student > Master 2 13%
Other 1 6%
Other 0 0%
Unknown 6 38%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 2 13%
Biochemistry, Genetics and Molecular Biology 2 13%
Nursing and Health Professions 1 6%
Agricultural and Biological Sciences 1 6%
Psychology 1 6%
Other 1 6%
Unknown 8 50%