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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Fluorometric CCHFV OTU protease assay with potent inhibitors
|
|---|---|
| Published in |
Virus Genes, July 2015
|
| DOI | 10.1007/s11262-015-1226-5 |
| Pubmed ID | |
| Authors |
Fatih Kocabas, Galip S. Aslan |
| Abstract |
Crimean-Congo hemorrhagic fever virus (CCHFV) is a deadly virus that has been listed in the Category C as a potential bioterror agent. There are no specific therapies against CCHFV, which urges identification of potential therapeutic targets and development of CCHFV therapies. CCHFV OTU protease takes an important role in viral invasion through antagonizing NF-κB signaling. Inhibition of CCHFV OTU protease by small molecules warrants an exciting potential as antiviral therapeutics. Here we report the expression and purification of a C-His-tagged recombinant CCHFV OTU protease in E. coli BL21 (DE3) host strain. Activity of the refolded purified recombinant viral OTU protease has been validated with a UB-AMC fluorescent assay. In addition, we show a dose-dependent inhibition of the viral OTU protease by two small molecules. This study provides a reliable approach for recombinant expression and purification of CCHFV OTU protease, and demonstrates validation of OTU protease activity and its inhibition based on a UB-AMC florescent assay. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Turkey | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 3% |
| Unknown | 33 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 21% |
| Researcher | 5 | 15% |
| Student > Postgraduate | 3 | 9% |
| Student > Bachelor | 2 | 6% |
| Other | 2 | 6% |
| Other | 6 | 18% |
| Unknown | 9 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 26% |
| Immunology and Microbiology | 3 | 9% |
| Medicine and Dentistry | 3 | 9% |
| Biochemistry, Genetics and Molecular Biology | 2 | 6% |
| Engineering | 2 | 6% |
| Other | 3 | 9% |
| Unknown | 12 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 August 2022.
All research outputs
#7,076,162
of 23,146,350 outputs
Outputs from Virus Genes
#163
of 980 outputs
Outputs of similar age
#81,527
of 262,930 outputs
Outputs of similar age from Virus Genes
#2
of 17 outputs
Altmetric has tracked 23,146,350 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 980 research outputs from this source. They receive a mean Attention Score of 2.4. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,930 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.