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Development and evaluation of near-isogenic lines for major blast resistance gene(s) in Basmati rice

Overview of attention for article published in Theoretical and Applied Genetics, April 2015
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Title
Development and evaluation of near-isogenic lines for major blast resistance gene(s) in Basmati rice
Published in
Theoretical and Applied Genetics, April 2015
DOI 10.1007/s00122-015-2502-4
Pubmed ID
Authors

Apurva Khanna, Vinay Sharma, Ranjith K. Ellur, Asif B. Shikari, S. Gopala Krishnan, U. D. Singh, G. Prakash, T. R. Sharma, Rajeev Rathour, Mukund Variar, S. K. Prashanthi, M. Nagarajan, K. K. Vinod, Prolay K. Bhowmick, N. K. Singh, K. V. Prabhu, B. D. Singh, Ashok K. Singh

Abstract

A set of NILs carrying major blast resistance genes in a Basmati rice variety has been developed. Also, the efficacy of pyramids over monogenic NILs against rice blast pathogen Magnaporthe oryzae has been demonstrated. Productivity and quality of Basmati rice is severely affected by rice blast disease. Major genes and QTLs conferring resistance to blast have been reported only in non-Basmati rice germplasm. Here, we report incorporation of seven blast resistance genes from the donor lines DHMASQ164-2a (Pi54, Pi1, Pita), IRBLz5-CA (Pi2), IRBLb-B (Pib), IRBL5-M (Pi5) and IRBL9-W (Pi9) into the genetic background of an elite Basmati rice variety Pusa Basmati 1 (PB1). A total of 36 near-isogenic lines (NILs) comprising of 14 monogenic, 16 two-gene pyramids and six three-gene pyramids were developed through marker-assisted backcross breeding (MABB). Foreground, recombinant and background selection was used to identify the plants with target gene(s), minimize the linkage drag and increase the recurrent parent genome (RPG) recovery (93.5-98.6 %), respectively, in the NILs. Comparative analysis performed using 50,051 SNPs and 500 SSR markers revealed that the SNPs provided better insight into the RPG recovery. Most of the monogenic NILs showed comparable performance in yield and quality, concomitantly, Pusa1637-18-7-6-20 (Pi9), was significantly superior in yield and stable across four different environments as compared to recurrent parent (RP) PB1. Further, among the pyramids, Pusa1930-12-6 (Pi2+Pi5) showed significantly higher yield and Pusa1633-7-8-53-6-8 (Pi54+Pi1+Pita) was superior in cooking quality as compared to RP PB1. The NILs carrying gene Pi9 were found to be the most effective against the concoction of virulent races predominant in the hotspot locations for blast disease. Conversely, when analyzed under artificial inoculation, three-gene pyramids expressed enhanced resistance as compared to the two-gene and monogenic NILs.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
India 1 2%
Unknown 63 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 13 20%
Student > Ph. D. Student 11 17%
Student > Master 5 8%
Student > Doctoral Student 3 5%
Student > Postgraduate 3 5%
Other 8 13%
Unknown 21 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 38 59%
Biochemistry, Genetics and Molecular Biology 2 3%
Environmental Science 1 2%
Unknown 23 36%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 September 2017.
All research outputs
#7,591,533
of 23,794,258 outputs
Outputs from Theoretical and Applied Genetics
#1,320
of 3,565 outputs
Outputs of similar age
#87,992
of 265,649 outputs
Outputs of similar age from Theoretical and Applied Genetics
#6
of 43 outputs
Altmetric has tracked 23,794,258 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 3,565 research outputs from this source. They receive a mean Attention Score of 4.9. This one has gotten more attention than average, scoring higher than 62% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,649 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 65% of its contemporaries.
We're also able to compare this research output to 43 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.