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Mendeley readers
Attention Score in Context
| Title |
Expansion of Intestinal Epithelial Stem Cells during Murine Development
|
|---|---|
| Published in |
PLOS ONE, November 2011
|
| DOI | 10.1371/journal.pone.0027070 |
| Pubmed ID | |
| Authors |
Jeffrey J. Dehmer, Aaron P. Garrison, Karen E. Speck, Christopher M. Dekaney, Laurianne Van Landeghem, Xiaofei Sun, Susan J. Henning, Michael A. Helmrath |
| Abstract |
Murine small intestinal crypt development is initiated during the first postnatal week. Soon after formation, overall increases in the number of crypts occurs through a bifurcating process called crypt fission, which is believed to be driven by developmental increases in the number of intestinal stem cells (ISCs). Recent evidence suggests that a heterogeneous population of ISCs exists within the adult intestine. Actively cycling ISCs are labeled by Lgr5, Ascl2 and Olfm4; whereas slowly cycling or quiescent ISC are marked by Bmi1 and mTert. The goal of this study was to correlate the expression of these markers with indirect measures of ISC expansion during development, including quantification of crypt fission and side population (SP) sorting. Significant changes were observed in the percent of crypt fission and SP cells consistent with ISC expansion between postnatal day 14 and 21. Quantitative real-time polymerase chain reaction (RT-PCR) for the various ISC marker mRNAs demonstrated divergent patterns of expression. mTert surged earliest, during the first week of life as crypts are initially being formed, whereas Lgr5 and Bmi1 peaked on day 14. Olfm4 and Ascl2 had variable expression patterns. To assess the number and location of Lgr5-expressing cells during this period, histologic sections from intestines of Lgr5-EGFP mice were subjected to quantitative analysis. There was attenuated Lgr5-EGFP expression at birth and through the first week of life. Once crypts were formed, the overall number and percent of Lgr5-EGFP positive cells per crypt remain stable throughout development and into adulthood. These data were supported by Lgr5 in situ hybridization in wild-type mice. We conclude that heterogeneous populations of ISCs are expanding as measured by SP sorting and mRNA expression at distinct developmental time points. |
Mendeley readers
The data shown below were compiled from readership statistics for 98 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 3% |
| Portugal | 1 | 1% |
| France | 1 | 1% |
| Unknown | 93 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 26 | 27% |
| Researcher | 23 | 23% |
| Student > Master | 12 | 12% |
| Student > Doctoral Student | 7 | 7% |
| Other | 6 | 6% |
| Other | 14 | 14% |
| Unknown | 10 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 44 | 45% |
| Biochemistry, Genetics and Molecular Biology | 18 | 18% |
| Medicine and Dentistry | 11 | 11% |
| Immunology and Microbiology | 5 | 5% |
| Veterinary Science and Veterinary Medicine | 2 | 2% |
| Other | 5 | 5% |
| Unknown | 13 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 March 2012.
All research outputs
#18,300,116
of 22,656,971 outputs
Outputs from PLOS ONE
#153,699
of 193,432 outputs
Outputs of similar age
#117,274
of 142,871 outputs
Outputs of similar age from PLOS ONE
#2,152
of 2,628 outputs
Altmetric has tracked 22,656,971 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 193,432 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.0. This one is in the 10th percentile – i.e., 10% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 142,871 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 8th percentile – i.e., 8% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 2,628 others from the same source and published within six weeks on either side of this one. This one is in the 7th percentile – i.e., 7% of its contemporaries scored the same or lower than it.