| Chapter title |
ChIP-Seq Analysis in Neurospora crassa
|
|---|---|
| Chapter number | 19 |
| Book title |
Fungal Genomics
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7804-5_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7803-8, 978-1-4939-7804-5
|
| Authors |
Aileen R. Ferraro, Zachary A. Lewis, Ferraro, Aileen R., Lewis, Zachary A. |
| Abstract |
Chromatin immunoprecipitation paired with next-generation sequencing (ChIP-seq) can be used to determine genome-wide distribution of transcriptions factors, transcriptional machinery, or histone modifications. DNA-protein interactions are covalently cross-linked with the addition of formaldehyde. Chromatin is prepared and sheared, then immunoprecipitated with the appropriate antibody. After reversal of cross-linking and treating with protease, the resulting DNA fragments are sequenced and mapped to the reference genome to determine overall enrichment. Here we describe a method of ChIP-seq for investigating protein-DNA interactions in the filamentous fungus Neurospora crassa. |
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