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Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD

Overview of attention for article published in International Brazilian Journal of Urology, January 2015
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Title
Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD
Published in
International Brazilian Journal of Urology, January 2015
DOI 10.1590/s1677-5538.ibju.2014.0400
Pubmed ID
Authors

Chenghe Wang, Zhong Chen, Jia Wu, Yan Zhang, Jia Hu, Qiangqiang Ge, Tao Wang, Weimin Yang, Hua Xu, Jihong Liu, Zhangqun Ye

Abstract

RNA activation (RNAa) is a mechanism of gene activation triggered by promoter-targeted small double stranded RNAs (dsRNAs), also known as small activating RNAs (saRNAs). Myogenic regulatory factor MyoD is regarded as the master activator of myogenic differentiation cascade by binding to enhancer of muscle specific genes. Stress urinary incontinence (SUI) is a condition primarily resulted from urethral sphincter deficiency. It is thus expected that by promoting differentiation of adipose-derived stem cells (ADSCs) into myoblasts by activating MyoD gene through RNAa may offer benefits to SUI. Rats ADSCs were isolated, proliferated in vitro, and identified by flow cytometry. Purified ADSCs were then transfected with a MyoD saRNA or control transfected. Real-time polymerase chain reaction (RT-PCR) and western blotting were used to detect MyoD mRNA and protein expression, respectively. Immunocytochemical staining was applied to determine the expression of desmin protein in transfected cells. Cell viability was measured by using CellTiter 96R AQueous One Solution Cell Proliferation Assay kit. Transfection of a MyoD saRNA (dsMyoD) into ADSCs significantly induced the expression of MyoD at both the mRNA and protein levels, and inhibited cell proliferation. Desmin protein expression was detected in dsMyoD treated ADSCs 2 weeks later. Our findings show that RNAa mediated overexpression of MyoD can promote transdifferentiation of ADSCs into myoblasts and may help treat stress urinary incontinence (SUI)-a condition primarily resulted from urethral sphincter deficiency.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 19%
Student > Ph. D. Student 2 13%
Other 1 6%
Lecturer 1 6%
Professor 1 6%
Other 2 13%
Unknown 6 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 19%
Agricultural and Biological Sciences 2 13%
Medicine and Dentistry 2 13%
Nursing and Health Professions 1 6%
Engineering 1 6%
Other 0 0%
Unknown 7 44%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 September 2015.
All research outputs
#18,034,109
of 26,370,291 outputs
Outputs from International Brazilian Journal of Urology
#368
of 734 outputs
Outputs of similar age
#226,272
of 363,478 outputs
Outputs of similar age from International Brazilian Journal of Urology
#33
of 59 outputs
Altmetric has tracked 26,370,291 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 734 research outputs from this source. They receive a mean Attention Score of 4.5. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 363,478 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 59 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.