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Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of β-nitrostyrene

Overview of attention for article published in Bioprocess and Biosystems Engineering, September 2015
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Title
Immobilization of Escherichia coli cells expressing 4-oxalocrotonate tautomerase for improved biotransformation of β-nitrostyrene
Published in
Bioprocess and Biosystems Engineering, September 2015
DOI 10.1007/s00449-015-1474-8
Pubmed ID
Authors

Lidija Djokic, Jelena Spasic, Sanja Jeremic, Branka Vasiljevic, Olivera Prodanovic, Radivoje Prodanovic, Jasmina Nikodinovic-Runic

Abstract

The enzyme 4-oxalocrotonate tautomerase (4-OT) encoded by the xylH gene is a part of the degradation pathway of aromatic compounds in Pseudomonas putida mt-2. 4-OT was described to catalyze Michael-type addition of acetaldehyde to β-nitrostyrene, and the whole cell system based on recombinantly expressed 4-OT has been developed previously. In this study biocatalytic process based on Escherichia coli whole cells expressing 4-OT was significantly improved using immobilization and ex situ product recovery strategies. Whole cell immobilization in alginate beads was applied in biocatalytic production of 4-nitro-3-phenyl-butanal from β-nitrostyrene and acetaldehyde. Immobilized biocatalyst showed wider pH activity range and could tolerate twofold higher initial concentrations of substrate in comparison to the free whole cell biocatalyst. Beads retained their initial activity over 10 consecutive biotransformations of the model reaction and remained suitable for the repetitive use with 85 % of the initial activity after two months of storage. Bioprocess was further improved by utilizing Amberlite XAD-2 hydrophobic resin for the product recovery. With this modification, the amount of organic solvent was reduced 40-fold in comparison to previously reported method making this biocatalytic process greener.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 8%
Unknown 11 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 42%
Student > Postgraduate 2 17%
Professor > Associate Professor 1 8%
Student > Ph. D. Student 1 8%
Unknown 3 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 25%
Chemistry 2 17%
Agricultural and Biological Sciences 2 17%
Immunology and Microbiology 1 8%
Environmental Science 1 8%
Other 0 0%
Unknown 3 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 September 2015.
All research outputs
#11,568,350
of 13,018,224 outputs
Outputs from Bioprocess and Biosystems Engineering
#375
of 548 outputs
Outputs of similar age
#204,656
of 247,865 outputs
Outputs of similar age from Bioprocess and Biosystems Engineering
#18
of 34 outputs
Altmetric has tracked 13,018,224 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 548 research outputs from this source. They receive a mean Attention Score of 2.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 34 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.