Title |
Roles of coinhibitory molecules B7-H3 and B7-H4 in esophageal squamous cell carcinoma
|
---|---|
Published in |
Tumor Biology, September 2015
|
DOI | 10.1007/s13277-015-4132-5 |
Pubmed ID | |
Authors |
Ling Wang, Na-na Cao, Shan Wang, Hong-wei Man, Peng-fei Li, Bao-en Shan |
Abstract |
The coinhibitory molecules, B7-H3 and B7-H4, have shown negative regulation in T cell activation and tumor-associated macrophage (TAM) polarization in tumor-specific immunity. Here, we investigated the expression of B7-H3 and B7-H4 in human and murine esophageal squamous cell carcinoma (ESCC) tissues to define their clinical significance and mechanism in a tumor microenvironment. In the present study, B7-H3 and B7-H4 were expressed in 90.6 and 92.7 % samples, respectively. High B7-H3 and B7-H4 expression was associated with advanced TNM stage and lymph node metastasis (p < 0.05, respectively). Patients with both B7-H3 and B7-H4 high-expressed tumors had the poorest prognosis (26.7 months), whereas those with both low-expressed tumors had the best survival (56.7 months). B7-H3 and B7-H4 expression were inclined to be positively related to the infiltration intensity of Treg cells and TAMs (p < 0.05, respectively), and B7-H3 expression is negatively associated with the intensity of CD8(+) T cells (p < 0.05). In 4-nitroquinoline 1-oxide (4-NQO)-induced murine models, high B7-H3 expression could only be detected at carcinoma stage, but abnormal B7-H4 expression appeared a little earlier at dysplasia stage. In vitro studies revealed that knockdown of B7-H3 on tumor cells suppressed ESCC cell migration and invasion, while knockdown of B7-H4 could inhibit ESCC cell growth. Overall, B7-H3 and B7-H4 are involved in ESCC progression and development and their coexpression could be valuable prognostic indicators. Interference of these negative regulatory molecules might be a new strategy for treating ESCC. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 24 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 17% |
Student > Bachelor | 3 | 13% |
Student > Master | 3 | 13% |
Student > Doctoral Student | 2 | 8% |
Student > Ph. D. Student | 2 | 8% |
Other | 5 | 21% |
Unknown | 5 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 7 | 29% |
Biochemistry, Genetics and Molecular Biology | 6 | 25% |
Immunology and Microbiology | 2 | 8% |
Agricultural and Biological Sciences | 2 | 8% |
Psychology | 1 | 4% |
Other | 1 | 4% |
Unknown | 5 | 21% |